IMG-EN-AS-72214-01.jpg
Assay-kits

SensoLyte® Thioflavin T ß-Amyloid (1-42) Aggregation Kit - 1 kit

$621.00
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  • Cat.Number : AS-72214
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The SensoLyte® ThT β-Amyloid (1-42) Aggregation kit provides a convenient and standard method to measure Aβ42 aggregation using Thioflavin T dye. Aβ42 peptide is pretreated to ensure it is in a monomeric state. An optimized fibrillation buffer is included with the kit, and two known inhibitors are supplied as controls. The assay is based on the property of ThT dye in which fluorescence (Ex/Em=440/484 nm) is increased when bound to aggregated Aβ peptides. Alzheimer's disease (AD), the most common cause of dementia, is characterized by the presence of senile plaques and neurofibrillary tangles, surrounded by damaged neurons. Beta-Amyloid (Aβ) peptides, Aβ40 (1-40) and Aβ42 (1-42), were found to be a major component of the above plaques. Many studies suggest that these peptides can form toxic oligomers and fibrils under physiological conditions and rapidly aggregate. Since Aβ aggregation is evidently an essential event in the pathogenesis of AD, a reliable assay is important to study Aβ fibrillation kinetics and screen for Aβ aggregation inhibitors.

Specifications

Packaging
Kits components
  • Component A: Assay Buffer: 25 ml Component B: Beta-Amyloid (1-42) (Aß42), human: 0.5 mg (2 x 0.25 mg, net peptide) Component C: Thioflavin T (ThT): 20 mM, 100 µl Component D: Morin: 20 mM, 25 µl Component E: Phenol Red: 20 mM, 25 µl
Chemistry
UniProt number
  • P05067
Properties
Absorbance (nm)
  • 440
Emission (nm)
  • 484
Storage & stability
Storage Conditions
  • Store kit components at 4°C. Components B, D, and E should be stored at -20°C if not used within one week
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

The Monofunctional Glycosyltransferase of Escherichia coli Localizes to the Cell Division Site and Interacts with Penicillin-Binding Protein 3, FtsW, and FtsN

J Bacteriol . 2018 Dec 31 ; 190(5) 1831-4 | DOI : PMC225871

  • Adeline Derouaux

Supratherapeutic concentrations of cilostazol inhibits β-amyloid oligomerization in vitro

Neurosci lett . 2018 Apr 21 ; 677 19-25 | DOI : 10.1016/j.neulet.2018.04.032

  • H. Shozawa

Do amyloid structures formed by Staphylococcus aureus phenol-soluble modulins have a biological function?

Int J Med Microbiol . 2017 Sep 01 ; 308(6) 675-682 | DOI : 10.1016/j.ijmm.2017.08.010

  • Y. Zheng

Neuroprotective Effect of Corydalis ternata Extract and Its Phytochemical Quantitative Analysis

Chem Pharm Bull . 2017 Jan 01 ; 65(9) 826-832 | DOI : 10.1248/cpb.c17-00300

  • Y.J. Kim

Novel Curcumin loaded nanoparticles engineered for Blood-Brain Barrier crossing and able to disrupt Abeta aggregates

Int J Pharm . 2017 May 08 ; 526(1-2) 413-424 | DOI : 10.1016/j.ijpharm.2017.05.015

  • R. Barbara

Microtubule modification influences cellular response to amyloid-β exposure

AIMS Biophysics . 2016 Jan 01 ; 3(2) 261-285 | DOI :  https://doi.org/10.3934/biophy.2016.2.261

  • N. Shamitko-Klingensmith

γ-Lactam alkaloids from the flower buds of daylily

J Natur med . 2016 Feb 05 ; 70(3) 376-383 | DOI : 10.1007/s11418-015-0963-z

  • T. Matsumoto

Chemical structures of constituents from the whole plant of Bacopa monniera

J Natur med . 2016 Mar 24 ; 70(3) 404-411 | DOI : 10.1007/s11418-016-0986-0

  • T. Ohta

Synapsin Condensates Recruit alpha-Synuclein

J Molec Biol . 2021 Jun 11 ; 433(12) 166961 | DOI : https://doi.org/10.1016/j.jmb.2021.166961

  • C. Hoffmann
  • et al

Humanin Blocks the Aggregation of Amyloid-β Induced by Acetylcholinesterase, an Effect Abolished in the Presence of IGFBP-3

Biochemistry . 2020 Jun 02 ; 59(21) 1981 | DOI : 10.1021/acs.biochem.0c00274

  • D. Price
  • et al

Human apolipoprotein E isoforms are differentially sialylated and the sialic acid moiety in ApoE2 attenuates ApoE2-Aβ interaction and Aβ fibrillation

Neurobiol Disease . 2022 Mar 01 ; 164 105631 | DOI : https://doi.org/10.1016/j.nbd.2022.105631

  • H-J Moon
  • et al

Combined Treatment with Curcumin and Ferulic Acid Suppressed the Aβ-Induced Neurotoxicity More than Curcumin and Ferulic Acid Alone

Int J Mol Sci. . 2022 Sep 01 ; 23(17) 9685 | DOI : 10.3390/ijms23179685

  • H. Ohashi
  • et al

Ficus erecta Thunb. Leaves Ameliorate Cognitive Deficit and Neuronal Damage in a Mouse Model of Amyloid-β-Induced Alzheimer’s Disease

Front. Pharmacol. . 2021 Apr 15 ; 12 607403 | DOI : https://doi.org/10.3389/fphar.2021.607403

  • E. Sohn
  • et al

Alleviatory effects of Danshen, Salvianolic acid A and Salvianolic acid B on PC12 neuronal cells and Drosophila melanogaster model of Alzheimer's disease

J Ethnopharmacol . 2021 Oct 28 ; 279 114389

  • F. Tan
  • et al

Galectin-3 promotes Aβ oligomerization and Aβ toxicity in a mouse model of Alzheimer’s disease

Cell Death Differ . 2020 Jan 01 ; 27(1) 192 | DOI : 10.1038/s41418-019-0348-z.

  • C-C. Tao
  • et al

Rational design of carbamate-based dual binding site and central AChE inhibitors by a “biooxidisable” prodrug approach: Synthesis, in vitro evaluation and docking studies

Eur. J Med Chem . 2018 Jul 15 ; 155 171 | DOI : https://doi.org/10.1016/j.ejmech.2018.05.057

  • M-L. Tints
  • et al

Neuroprotective Activity of Mentha Species on Hydrogen Peroxide-Induced Apoptosis in SH-SY5Y Cells

Nutrients . 2020 May 10 ; 12(5) 1366 | DOI : https://doi.org/10.3390/nu12051366

  • DM. Hanafy
  • et al

The Curcumin Derivative GT863 Protects Cell Membranes in Cytotoxicity by Aβ Oligomers

Int. J. Mol. Sci. . 2023 Feb 04 ; 24(4) 3089 | DOI : https://doi.org/10.3390/ijms24043089

  • Y. Momma
  • et al

Novel Hominid-Specific IAPP Isoforms: Potential Biomarkers of Early Alzheimer’s Disease and Inhibitors of Amyloid Formation

Biomolecules . 2023 Jan 13 ; 13(1) 167 | DOI : https://doi.org/10.3390/biom13010167

  • Q-R Liu
  • et al

Licochalcone E, a β-Amyloid Aggregation Inhibitor, Regulates Microglial M1/M2 Polarization via Inhibition of CTL1-Mediated Choline Uptake

Biomolecules . 2023 Jan 17 ; 13(2) 191 | DOI : https://doi.org/10.3390/biom13020191

  • E. Muto
  • et al