IMG-EN-AS-72213-01.jpg
Assay-kits

SensoLyte® Thioflavin T ß-Amyloid (1-40) Aggregation Kit - 1 kit

$652.00
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  • Cat.Number : AS-72213
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The SensoLyte® ThT β-Amyloid (1-40) Aggregation kit provides a convenient and standard method to measure Aβ40 aggregation using Thioflavin T dye. Aβ40 peptide is pretreated to ensure it is in a monomeric state. An optimized fibrillation buffer is included with the kit, and two known inhibitors are supplied as controls. The assay is based on the property of ThT dye in which fluorescence (Ex/Em=440/484 nm) is increased when bound to aggregated Aβ peptides. Alzheimer's disease (AD), the most common cause of dementia, is characterized by the presence of senile plaques and neurofibrillary tangles, surrounded by damaged neurons. Beta-Amyloid (Aβ) peptides, Aβ40 (1-40) and Aβ42 (1-42), were found to be a major component of the above plaques. Many studies suggest that these peptides can form toxic oligomers and fibrils under physiological conditions and rapidly aggregate. Since Aβ aggregation is evidently an essential event in the pathogenesis of AD, a reliable assay is important to study Aβ fibrillation kinetics and screen for Aβ aggregation inhibitors.

Specifications

Packaging
Kits components
  • Component A: Assay Buffer: 25 ml Component B: Beta-Amyloid (1-40) (Aß40), human: 0.5 mg (2 x 0.25 mg, net peptide) Component C: Thioflavin T (ThT): 20 mM, 100 µl Component D: Morin: 10 mM, 25 µl Component E: Phenol Red: 10 mM, 25 µl
Chemistry
UniProt number
  • P05067
Properties
Absorbance (nm)
  • 440
Emission (nm)
  • 484
Storage & stability
Storage Conditions
  • Store kit components at 4°C. Components B, D, and E should be stored at -20°C if not used within one week
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Humanin Blocks the Aggregation of Amyloid-β Induced by Acetylcholinesterase, an Effect Abolished in the Presence of IGFBP-3

Biochemistry . 2020 Jun 02 ; 59(21) 1981 | DOI : 10.1021/acs.biochem.0c00274

  • D. Price
  • et al

Combined Treatment with Curcumin and Ferulic Acid Suppressed the Aβ-Induced Neurotoxicity More than Curcumin and Ferulic Acid Alone

Int J Mol Sci. . 2022 Sep 01 ; 23(17) 9685 | DOI : 10.3390/ijms23179685

  • H. Ohashi
  • et al

The Curcumin Derivative GT863 Protects Cell Membranes in Cytotoxicity by Aβ Oligomers

Int. J. Mol. Sci. . 2023 Feb 04 ; 24(4) 3089 | DOI : https://doi.org/10.3390/ijms24043089

  • Y. Momma
  • et al