SensoLyte® Thioflavin T [Pyr3] β-Amyloid (3-42) Aggregation Kit - 1 Kit

Pyroglutamate modification of β-amyloid is one of the predominant amyloid peptide structures deposited in the human brain of Alzheimer’s disease and Down’s syndrome patients. The Pyro-Glu modification on the N-terminal of β-amyloid at position 3 provides resistance to degradation. It also has higher toxicity and aggregation propensity compared to non-modified beta-amyloid. Many studies suggest that this peptide can form toxic oligomers and fibrils under physiological conditions and rapidly aggregate. Since [Pyr3]-Aβ (3-42) aggregation is evidently an essential event in the pathogenesis of AD, a reliable assay is important to study Aβ fibrillation kinetics and screen for Aβ aggregation inhibitors.

SensoLyte® ThT [Pyr3]-Aβ (3-42) Aggregation kit provides a convenient and standard method to measure [Pyr3]-Aβ (3-42) aggregation using Thioflavin T dye. The peptide, provided in the kit, is pretreated to ensure it is in a monomer state. Optimized fibrillation buffer is included with the kit, and two known inhibitors are supplied as a control. The assay is based on the property of ThT dye to increase its fluorescence Ex/Em=440/484 nm when bound to aggregated Aβ peptides.

Specifications