As a world leader in FRET (fluorescence resonance energy transfer) peptide technologies, we propose a full range of dye-quencher pairs for customized assay developments.
Our dye/quencher labeling offer
We offer a full-range of dye-quencher FRET pairs and fluorogenic substrates.
The tables below list the dyes and quenchers available to label your custom peptides
|Dye (donor)||Quencher (acceptor)||Donor Ex/Em|
|EDANS||DABCYL, DABCYL Plus, QXL®490||
|HiLyte™ Fluor 488||QXL®520||
|CyLyte Fluor 3||QXL®570||
|HiLyte™ Fluor 555||QXL®570||
|CyLyte Fluor 5||QXL®670||
|HiLyte™ Fluor 647||QXL®670||
|CyLyte Fluor 7||IR-QXL®||
QXL® and Dye pairings are available from our custom labeling services & from our catalog SensoLyte® protease activity assay kits
(ie., QXL® 520 - HiLyte™ Fluor 488, QXL® 520 - FAM)
FRET-based trilateration of probes bound within functional ryanodine receptors.
Bengt Svensson,Tetsuro Oda, Florentin R.Nitu, Yi Yang, Iustin Cornea, Ye Chen-Izu, James D.Fessenden, Donald M.Bers, David D.Thomas, Razvan L.Cornea1
Biophysical journal. 2014 Nov 4;107(9):2037-48. DOI: https://doi.org/10.1016/j.bpj.2014.09.029
From AnaSpec: DPc10 peptides either unlabeled, or labeled with HiLyte Fluor 647 (HF647)
AMCA to TAMRA long range resonance energy transfer on a flexible peptide.
A. Synak, R.Fudala, I.Gryczynski, L.Kułak, S.Shah, I. E. Serdiuk, B. Grobelna, P.Arłukowicz, A.Kubicki, P.Bojarski
Dyes and Pigments. 2018 Nov;158:60-64
From AnaSpec: Lys(AMCA)-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys(TAMRA)-NH2 peptide
Why use FRET?
FRET and fluorogenic substrates are more sensitive than chromogenic substrates with linear dynamic range and great reproducibility. FRET-based peptides are used in a variety of applications, with high benefit when used as protease substrates in high-throughput inhibitor screening & drug discovery.
Using Dyes and Quenchers from AnaSpec offers many advantages:
- Unique collection of proprietary quenchers (QXL®) which tandem with the most popular dyes
- Top quality HiLyte™ Fluor dyes
- Access to the Cy dyes at an affordable price (CyLyte dyes which have identical structures to the trademarked Cy®)
- Longer wavelengths for improved sensitivities
- Easy adaptation to high throughput screening assays
- Protease activity detection & measurement
- Protease-based drug target screening and discovery
- High throughput screening of protease inhibitors
FRET occurs when a donor (fluorophore) and an acceptor (quencher) are in close proximity (within 10-100Å from each other). The energy from the excited fluorophore is transfered to the quencher resulting in no fluorescence emission.
If the donor and acceptor are on a same peptide which is a protease substrate, enzymatic hydrolysis will result in spatial separation of the donor from the acceptor, and fluorescence emission.