Our dye/quencher labeling offer

We offer a full-range of dye-quencher FRET pairs and fluorogenic substrates.
The tables below list the dyes and quenchers available to label your custom peptides

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FRET Pairs
Dye (donor) Quencher (acceptor) Donor Ex/Em
Mca Dnp

 325/393 nm

EDANS DABCYL, DABCYL Plus, QXL®490

 335/493 nm

FAM QXL®520

 492/518 nm

FITC QXL®520

 494/519 nm

HiLyte™ Fluor 488 QXL®520

 502/527 nm

CyLyte Fluor 3 QXL®570

 550/564 nm

HiLyte™ Fluor 532 QXL®570

 545/565 nm

HiLyte™ Fluor 555 QXL®570

 550/566 nm

TAMRA QXL®570

 541/568 nm

Rox QXL®610

 568/591 nm

CyLyte Fluor 5 QXL®670

 648/663 nm

HiLyte™ Fluor 647 QXL®670

 650/675 nm

CyLyte Fluor 7 IR-QXL®

 750/773 nm


QXL® and Dye pairings are available from our custom labeling services & from our catalog SensoLyte® protease activity assay kits
(ie., QXL® 520 - HiLyte™ Fluor 488, QXL® 520 - FAM)

Why use FRET?

FRET and fluorogenic substrates are more sensitive than chromogenic substrates with linear dynamic range and great reproducibility. FRET-based peptides are used in a variety of applications, with high benefit when used as protease substrates in high-throughput inhibitor screening & drug discovery.

Benefits

Using Dyes and Quenchers from AnaSpec offers many advantages:

  • Unique collection of proprietary quenchers (QXL®) which tandem with the most popular dyes
  • Top quality HiLyte™ Fluor dyes
  • Access to the Cy dyes at an affordable price (CyLyte dyes which have identical structures to the trademarked Cy®)
  • Longer wavelengths for improved sensitivities
  • Easy adaptation to high throughput screening assays

Applications

  • Protease activity detection & measurement
  • Protease-based drug target screening and discovery
  • High throughput screening of protease inhibitors