IMG-EN-AS-71303-01.jpg
Assay-kits

SensoLyte® Cell Cytotoxicity Assay Kit Fluorimetric, Large size - 1 kit

$1,145.00
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  • Cat.Number : AS-71303
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

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The damage of cell membrane leads to the release of cytoplasmic enzymes. The measurement of released cytoplasmic lactate dehydrogenase (LDH) is a well-accepted assay to estimate cell membrane integrity and quantify cytotoxicity. The SensoLyte® Cell Cytotoxicity Assay Kit uses resazurin as a sensitive fluorogenic indicator (Ex/Em=560 nm/590 nm upon conversion) to measure LDH activity. The assay can be performed in a mixed population of damaged and viable cells, but it only measures the LDH released from damaged cells. The cytoplasmic LDH in living cells produces little signals under assay condition. There is no need for extra steps to separate living cells and supernatant. The fluorescent signal is proportional to the number of damaged cells (up to 2.5X104 cell, r2>0.95) with the detection limit reaching 100 dead cells. The kit is suitable for high throughput screening of cytotoxicity of a variety of compounds. 384-well or 1536-well format can be used with minor modifications.

Specifications

Packaging
Kits components
  • Component A: Assay mixture 10 bottles Component B: Assay buffe:r 25 mL X 10 bottles Component C: Lysis solution: 100 mL Component D: Stop solution: 100 mL
Properties
Absorbance (nm)
  • 560
Emission (nm)
  • 590
Storage & stability
Storage Conditions
  • Store components A and B at -20°C. Components C and D can be stored at room temperature for convenience.
Activity
Application
Detection Method
Detection Limit
  • 100 cells
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

In vitro cytotoxicity screening of water-dispersible metal oxide nanoparticles in human cell lines

Bioprocess Biosyst Eng. . 2009 Jul 28 ; 33(1) 21 | DOI : 10.1007/s00449-009-0354-5

  • J. Choi

Diminished survival of human cytotrophoblast cells exposed to hypoxia/reoxygenation injury and associated reduction of heparin-binding epidermal growth factor-like growth factor.

Am J Obstet Gynecol. . 2008 Apr 01 ; 198(4) 471. e1 | DOI : 10.1016/j.ajog.2008.01.009

  • R.E. Leach

Epidermal Growth Factor-Like Growth Factors Prevent Apoptosis of Alcohol-Exposed Human Placental Cytotrophoblast Cells

Biol Reprod. . 2007 Aug 23 ; 77(1) 53 | DOI : 10.1095/biolreprod.106.057984

  • G.S. Wolff

Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor.

Development. . 2006 Jan 11 ; 133(4) 751 | DOI : 10.1242/dev.02237

  • D.R. Armant

An experimental study of rabbit conjunctival epithelial toxicity using co-treatment with Mitomycin-C and a histone deacetylase inhibitor

Arch Pharmacal Res . 2010 Aug 28 ; 33 1261 | DOI : https://doi.org/10.1007/s12272-010-0817-x

  • T.H. Kim

References

A new, simple, nonradioactive, nontoxic in vitro assay to monitor corneal endothelial cell viability.

Invest Ophthalmol Vis . 1997 Sep 01 ; 38(10) 1929

  • EM. Larson
  • et al

Assessment of a simple, non-toxic Alamar blue cell survival assay to monitor tomato cell viability

Phytochem Anal . 2001 Sep 01 ; 12(5) 340 | DOI : https://doi.org/10.1002/pca.595

  • H. Byth
  • et al

A new nondestructive cytometric assay based on resazurin metabolism and an organ culture model for the assessment of corneal viability

Cytometry . 2003 Aug 18 ; 55A(1) 7 | DOI : https://doi.org/10.1002/cyto.a.10067

  • S. Perrot
  • et al