Existing Account

Please login first to complete purchase/ quotation request, view custom order reports, or create favorites list.

Customer ID:
Stay Logged In

Forgot your Customer ID or Password?
New Account

Don't have an account with us yet? Please set up an account to place order or obtain customer services.

Assay Kits  >  Cathepsin Assay Kits  >>  SensoLyte ® 520 Cathepsin L Assay Kit *Fluorimetric*

Product Name SensoLyte ® 520 Cathepsin L Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-72218
US$ $504

Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin L, a lysosomal endopeptidase, is a member of the papain-like family of cysteine proteinases. It is involved in the promotion of tumor cell invasion, antigen processing and turnover of intracellular and secreted proteins.

The SensoLyte® 520 Cathepsin L Activity Assay Kit uses a QXL® 520/HiLyte™ Fluor 488 labeled FRET peptide substrate for measuring enzyme activity. The long wavelength FRET substrate is designed based on a sequence surrounding the cleavage site of Cathepsin L. The QXL® 520/HiLyte™ Fluor 488 pair is used for optimal quenching of the intact substrate. When active Cathepsin L cleaves the FRET substrate, it results in an increase of HiLyte™ Fluor 488 fluorescence and is monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, the optimal pH for Cathepsin L activity.

Kit size: 100 assays (96-well plate)

Images SensoLyte ® 520 Cathepsin L Assay Kit *Fluorimetric*
Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20°C
  < Back