ASSAY KITS

Adhesion and ECM Assay Kits

Matrix metalloproteinases (MMPs)

MMPs belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components.1-2

The family currently includes more than 25 members that can be divided into collagenases (MMP-1, -8, and -13), gelatinases (MMP-2 and 9), stromelysins (MMP-3 and 10), matrilysins (MMP-7 and 26), and the membrane-type MMPs (MMP-14 to 17 and 24).

Collectively, MMPs cleave most, if not all, of the constituents of the extracellular matrix (ECM) and are involved in the breakdown and remodeling of many tissues and organs.

They preferentially cleave specific substrate sequences, but most often, one substrate can be cleaved by several MMP members.

AnaSpec is providing a comprehensive list of SensoLyte® assay kits to monitor the activity of MMPs and other adhesion-related proteases.



References

1. Woessner, J. et al. J. Biol. Chem. 263, 16918 (1988)
2. Woessner, J. FASEB J. 5, 2145 (1991)

FRET substrates

FRET occurs between a peptide tagged to a donor and an acceptor when placed within 10-100Å of each other resulting in the donor’s excitation fluorescence to be quenched by the acceptor. Enzymatic hydrolysis of the peptide results in recovery of the donor fluorescence following spatial separation of the donor and acceptor upon energy transfer.

Dye (donor) Quencher (acceptor) Donor Ex/Em
SensoLyte® 390 Mca Dnp

 325/393 nm

SensoLyte® 490 EDANS DABCYL

 340/490 nm

SensoLyte® 520 5-FAM or HiLyte™ Fluor 488 QXL®520

 494/521 nm

SensoLyte® 570 5-TAMRA QXL®570

 547/574 nm

 

Fluorogenic substrates

These substrates do not require a quencher and contain a C-terminal dye that does not fluoresce until it is cleaved from the peptide (fluorescent form of dye is released).

Dye (donor) Donor Ex/Em
SensoLyte® AMC AMC

 351/430 nm

SensoLyte® AFC AFC

 382/480 nm

SensoLyte® Rh110 Rh110

 501/527 nm

  • Catalog peptides

    We have adhesion and ECM peptides ready for immediate shipment

    View our collection

  • Recombinant Proteins

    We produce MMPs recombinant proteins and catalytic domains

    View our collection