Labeling-detection

5-FAM, SE [5-Carboxyfluorescein, SE]

$134.00
Check your price
  • Cat.Number : AS-81007
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

Alternative choices

Size

Quantity

Although FITC reagents have been more often used to prepare fluorescent bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents generate carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions, give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.
5-FAM is a single isomer. It is one of the most popular green fluorescent reagents used for in situ labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules.

Specifications

Chemistry
CAS registry number
  • 92557-80-7
Molecular Formula
  • C25H15NO9
Molecular Mass/ Weight
  • 473.4
Properties
Absorbance (nm)
  • 492
Emission (nm)
  • 518
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

You may also be interested in the following product(s)

IMG-EN-AS-20151-01.jpg

5-FITC [Fluorescein-5-isothiocyanate] - 1 g

Cat.Number : AS-20151
$180.00 Excl. Tax
IMG-EN-AS-72047-01.jpg

AnaTag™ HiLyte™ Fluor 488 Protein Labeling Kit - 1 kit

Cat.Number : AS-72047
$454.00 Excl. Tax
IMG-EN-AS-81161-1-01.jpg

HiLyte™ Fluor 488 succinimidyl ester (SE)

Cat.Number : AS-81161-1
$183.00 Excl. Tax

References

Application of terminal RFLP analysis to characterize oral bacterial flora in saliva of healthy subjects and patients with periodontitis

J Med Microbiol . 2003 Jan 01 ; 52(1) 79 | DOI : https://doi.org/10.1099/jmm.0.04991-0

  • M. Sakamoto

Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis

Electrophoresis . 2001 Aug 01 ; 22(13) 2691 | DOI : 10.1002/1522-2683(200108)22:13<2691::AID-ELPS2691>3.0.CO;2-S

  • M. Hahn
  • et al