5-FAM, SE [5-Carboxyfluorescein, SE]
- Cat.Number : AS-81007
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Although FITC reagents have been more often used to prepare fluorescent bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents generate carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions, give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.
5-FAM is a single isomer. It is one of the most popular green fluorescent reagents used for in situ labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules.
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References
Application of terminal RFLP analysis to characterize oral bacterial flora in saliva of healthy subjects and patients with periodontitis
J Med Microbiol . 2003 Jan 01 ; 52(1) 79 | DOI : https://doi.org/10.1099/jmm.0.04991-0
- M. Sakamoto
Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis
Electrophoresis . 2001 Aug 01 ; 22(13) 2691 | DOI : 10.1002/1522-2683(200108)22:13<2691::AID-ELPS2691>3.0.CO;2-S
- M. Hahn
- et al