Labeling-detection

5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, SE]

$928.00
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  • Cat.Number : AS-81006-1000
  • Availability :
    In production
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Although FITC reagents have been more often used to prepare fluorescent bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents generate carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions, give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.

Specifications

Chemistry
CAS registry number
  • 76608-16-7
Molecular Formula
  • C25H15NO9
Molecular Mass/ Weight
  • 473.4
Properties
Absorbance (nm)
  • 494
Emission (nm)
  • 519
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO (soluble at 1 mmol in 1ml DMF)
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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References

Dye structure affects Taq DNA polymerase terminator selectivity

Nucleic Acids Res . 1999 Apr 01 ; 27(8) 1912 | DOI : https://doi.org/10.1093/nar/27.8.1912

  • JW. Brandis
  • et al

Factor V Leiden genotyping using real-time fluorescent polymerase chain reaction

Mol Cell Probes . 2000 Aug 01 ; 14(4) 249 | DOI : 10.1006/mcpr.2000.0313

  • SJ. Sanders
  • et al

Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis

Electrophoresis . 2001 Aug 01 ; 22(13) 2691 | DOI : 10.1002/1522-2683(200108)22:13<2691::AID-ELPS2691>3.0.CO;2-S

  • M. Hahn
  • et al