Labeling-detection

AnaTag™ APC Labeling Kit - 1 kit

$564.00
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  • Cat.Number : AS-72111
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    In stock
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The AnaTag™ APC Labeling Kit is optimized for use in the conjugation of allophycocyanin (APC) to antibodies. APC is an ultra-sensitive, near-infrared fluorescent tracer with a high quantum yield (Ex/Em = 650 nm/660 nm). APC- labeled antibodies are used in applications such as flow cytometry and immunofluorescence. The AnaTagTM APC Labeling Kit contains SH-reactive APC. SMCC modified allophycocyanin reacts with the thiol groups of target antibody without the need for additional activation, thus simplifying the conjugation protocol. AnaTagTM APC Labeling Kit contains a chemically cross-linked APC (CL-APC) that is much more stable than the native APC, but still retains the original spectroscopic properties. The amount of APC supplied in this kit is sufficient for labeling up to 1 mg of antibody. The kit provides all reagents, purification columns and a detailed step-by-step protocol. Cross-linked and SMCC-activated APC are also available as separate products. Kit Size: 1 labeling reaction (1x1 mg IgG)
Note: AnaTag kits are not intended for peptide labeling.

Specifications

Packaging
Kits components
  • Component A: Activated CL-APC (SMCC activated cross-linked allophycocyanin): 2 mg, 10mg/mL Component B: DTT, reduces antibody: 0.1 mL Component C: Spin column which desalts protein by centrifugation: 1 column Component D: Wash tube to collect buffer from the spin column: 1 tube Component E: Collection tube to collect sample from the spin column: 1 tube Component F: Gravity column which desalts protein by gravity: 1 column Component G: DMSO: 1 mL Component H: NEM which blocks free sulfhydryl groups: 1 vial Component I: Reaction buffer for conjugation of APC with protein: 100 mL
Properties
Absorbance (nm)
  • 650
Emission (nm)
  • 660
Storage & stability
Storage Conditions
  • Store all components at 4°C. Protect component A from light and do not freeze.Components B and H may be frozen for best performance.
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

The nutraceutical flavonoid luteolin inhibits ADAMTS-4 and ADAMTS-5 aggrecanase activities

J Mol Med . 2011 Mar 02 ; 89(6) 611 | DOI : 10.1007/s00109-011-0741-7

  • A. Moncada-Pazos