The AnaTag™ APC Labeling Kit is optimized for use in the conjugation of allophycocyanin (APC) to antibodies. APC is an ultra-sensitive, near-infrared fluorescent tracer with a high quantum yield (Ex/Em = 650 nm/660 nm). APC- labeled antibodies are used in applications such as flow cytometry and immunofluorescence. The AnaTagTM APC Labeling Kit contains SH-reactive APC. SMCC modified allophycocyanin reacts with the thiol groups of target antibody without the need for additional activation, thus simplifying the conjugation protocol. AnaTagTM APC Labeling Kit contains a chemically cross-linked APC (CL-APC) that is much more stable than the native APC, but still retains the original spectroscopic properties. The amount of APC supplied in this kit is sufficient for labeling up to 1 mg of antibody. The kit provides all reagents, purification columns and a detailed step-by-step protocol. Cross-linked and SMCC-activated APC are also available as separate products. Kit Size: 1 labeling reaction (1x1 mg IgG) Note: AnaTag kits are not intended for peptide labeling.
Component A: Activated CL-APC (SMCC activated cross-linked allophycocyanin): 2 mg, 10mg/mL
Component B: DTT, reduces antibody: 0.1 mL
Component C: Spin column which desalts protein by centrifugation: 1 column
Component D: Wash tube to collect buffer from the spin column: 1 tube
Component E: Collection tube to collect sample from the spin column: 1 tube
Component F: Gravity column which desalts protein by gravity: 1 column
Component G: DMSO: 1 mL
Component H: NEM which blocks free sulfhydryl groups: 1 vial
Component I: Reaction buffer for conjugation of APC with protein: 100 mL
Storage & stability
Store all components at 4°C. Protect component A from light and do not freeze.Components B and H may be frozen for best performance.