Labeling-detection

B-PE (B-Phycoerythrin) - 1 mg

  • Cat.Number : AS-82001
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    In stock
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B-Phycoerythrin (B-PE), a fluorescent protein from phycobiliprotein family, is isolated from cyanobacteria and eukaryotic algae. Its primary absorption peak is at 545 nm with a secondary peak at 563 nm. B-PE consists of α, β and γ subunits and is present as (αβ)6γ. B-PE and the closely related R-PE are the most intensely fluorescent phycobiliproteins having orange fluorescence. They are significantly brighter and more photostable than conventional organic fluorophores. B-PE labeled streptavidin, primary and secondary antibodies have been widely used in applications such as flow cytometry and multi-color immunofluorescent staining.

Specifications

Chemistry
Molecular Mass/ Weight
  • 240000
Properties
Absorbance (nm)
  • 545
Emission (nm)
  • 578
Color
Storage & stability
Form
  • In solution
Resuspension condition
  • Before use, centrifuge the B-PE suspension at 10,000g for 10 min at 4°C. Discard the supernatant. Resuspend the pellet (B-PE) into the desired buffer. Desalt the sample using either Sephadex G-25 or dialysis.
Storage Buffer
  • Sodium phosphate buffer, pH 7.0 with ammonium sulfate
Storage Conditions
  • Store at 4°C and protect from light. DO NOT FREEZE
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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References

Characterization of digoxigenin-labeled B-phycoerythrin by capillary electrophoresis with laser-induced fluorescence. Application to homogeneous digoxin immunoassay

J Chromatogr . 1994 Jan 10 ; 680(2) 425

  • FT. Chen
  • SL Pentoney

Isolation, crystallization, crystal structure analysis and refinement of B-phycoerythrin from the red alga Porphyridium sordidum at 2.2 Å resolution

J Mol Biol . 1992 Dec 05 ; 228(3) 935 | DOI : https://doi.org/10.1016/0022-2836(92)90876-L

  • R. Ficner
  • et al