Labeling-detection

R-PE (R-Phycoerythrin) - 1 mg

  • Cat.Number : AS-82004
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    In stock
  • Shipping conditions : Ice fees will apply

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R-Phycoerythrin (R-PE), a fluorescent protein from phycobiliprotein family, is isolated from red algae. Its primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. The broad excitation spectrum provides the advantage for multi-color immunofluorescent staining or cell sorting. R-PE consists of α, β and γ subunits and is present as (αβ)6γ. R-PE and the closely related B-PE are the most intensely fluorescent phycobiliproteins having orange fluorescence. They are significantly brighter and more photostable than conventional organic fluorophores.

Specifications

Chemistry
Molecular Mass/ Weight
  • 240000
Properties
Absorbance (nm)
  • 565
Emission (nm)
  • 578
Color
Storage & stability
Form
  • In solution
Resuspension condition
  • Before use, centrifuge the R-PE suspension at 10,000g for 10 min at 4°C. Discard the supernatant. Resuspend the pellet (R-PE) into the desired buffer. Desalt the sample using either Sephadex G-25 or dialysis.
Storage Buffer
  • Sodium phosphate buffer, pH 7.0 with ammonium sulfate
Storage Conditions
  • Store at 4°C and protect from light. DO NOT FREEZE
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

AID-expressing germinal center B cells cluster normally within lymph node follicles in the absence of FDC-M1+ CD35+ follicular dendritic cells but dissipate prematurely.

J Innumol . 2013 Sep 25 ; 191(9) 4521 | DOI : 10.4049/​jimmunol.1300769

  • B. Boulianne

AID-expressing germinal center B cells cluster normally within lymph node follicles in the absence of FDC-M1+ CD35+ follicular dendritic cells but dissipate prematurely.

J Innumol . 2013 Sep 25 ; 191(9) 4521 | DOI : 10.4049/​jimmunol.1300769.

  • B. Boulianne

References

The experimental research of R-phycoerythrin subunits on cancer treatment: a new photosensitizer in PDT

Cancer Biother Radiopharm . 2004 Jul 05 ; 17(1) 35 | DOI : https://doi.org/10.1089/10849780252824055

  • H. Bei
  • et al

One-step purification of R-phycoerythrin from the red macroalga Palmaria palmata using preparative polyacrylamide gel electrophoresis.

J Chromatogr B Biomed Sci Appl . 2000 Feb 28 ; 739(1) 117 | DOI : https://doi.org/10.1016/S0378-4347(99)00433-8

  • AV. Galland-Irmouli
  • et al