Labeling-detection

SMCC Activated B-PE (B-Phycoerythrin) - 1 mg

$154.00
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  • Cat.Number : AS-72109
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    In stock
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B-Phycoerythrin (B-PE), a fluorescent protein from phycobiliprotein family, is isolated from cyanobacteria and eukaryotic algae. SMCC activated B-PE is chemically modified with SMCC. SMCC reacts with the primary amine on B-PE and introduces maleimide groups to B-PE. These maleimide groups easily react with thiol groups of target protein without the need for any additional activation, resulting in convenient conjugation of B-PE with proteins. Its primary absorption peak is at 545 nm with secondary peak at 563 nm. B-PE and the closely related R-PE are the most intensely fluorescent phycobiliproteins having orange fluorescence. They are significantly brighter and more photostable than conventional organic fluorophores. B-PE conjugates have been widely used in applications such as flow cytometry and multi-color immunofluorescent staining.

Specifications

Properties
Absorbance (nm)
  • 545
Emission (nm)
  • 578
Color
Quantity & Purity
Concentration
  • 1 mg/ml
Storage & stability
Form
  • In solution
Storage Buffer
  • MES buffer, pH 6.0 with EDTA and preservative
Storage Conditions
  • Store at 4 °C and protect from light The product is stable for 1 year. DO NOT FREEZE.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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References

Characterization of digoxigenin-labeled B-phycoerythrin by capillary electrophoresis with laser-induced fluorescence. Application to homogeneous digoxin immunoassay

J Chromatogr . 1994 Jan 10 ; 680(2) 425

  • FT. Chen
  • SL Pentoney

Isolation, crystallization, crystal structure analysis and refinement of B-phycoerythrin from the red alga Porphyridium sordidum at 2.2 Å resolution

J Mol Biol . 1992 Dec 05 ; 228(3) 935 | DOI : https://doi.org/10.1016/0022-2836(92)90876-L

  • R. Ficner
  • et al