IMG-EN-AS-63717-01.jpg
Peptides

Bacterial Sortase Substrate III, Abz/DNP - 1 mg

$290.00
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  • Cat.Number : AS-63717
  • Availability :
    In stock

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The Staphylococcus aureus transpeptidase Sortase A (SrtA) anchors virulence and colonization-associated surface proteins to the cell wall. SrtA selectively recognizes a C-terminal LPXTG motif. SrtA readily reacts with its native substrate Abz-LPETG-Dap(DNP)-NH2, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this FRET substrate can be monitored at Ex/Em=320 nm/420 nm.

Specifications

Chemistry
Sequence one letter code
  • Abz-LPETG-K(Dnp)-NH2
Sequence three letter code
  • Abz-Leu-Pro-Glu-Thr-Gly-Lys(Dnp)-NH2
Molecular Mass/ Weight
  • 928
Properties
Absorbance (nm)
  • 320
Emission (nm)
  • 420
Modification
Conjugation type
  • Double dyes
Modification Name
Conjugation
  • Conjugated
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Storage Conditions
  • - 20 °C Protected from light
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • bacteria

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References

Engineering the Substrate Specificity of Staphylococcus aureus Sortase A

J Biol Chem . 2007 Mar 01 ; 282(9) 6571 | DOI : https://doi.org/10.1074/jbc.M610519200

  • M. Bentley
  • et al