IMG-EN-AS-25263-5-01.jpg
Peptides

Caspase 3 (Apopain) Substrate 1, chromogenic - 5 mg

$110.00
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  • Cat.Number : AS-25263-5
  • Availability :
    In stock

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Caspase-1 substrate with Km = 18 uM and kcat = 0.5 M-1s-1; Caspase-3 substrate with Km = 11 uM and kcat = 2.4 M-1s-1;Caspase-4 substrate with Km = 32 uM and kcat = 0.05 M-1s-1 Caspase-6 substrate with Km = 180 uM and kcat = 0.6 M-1s-1 ; Caspase-7 substrate with Km = 12 uM and kcat = 0.4 M-1s-1; Caspase-8 substrate with Km = 167 uM;
pNA (4-nitroaniline)-derived caspase substrates are widely used for the colorimetric detection of various caspase activities. Cleavage of pNA peptides by caspases generates pNA that is monitored colorimetrically at ~405 nm. pNA has maximum absorption around 408 nm.

Specifications

Chemistry
Sequence one letter code
  • Ac-DEVD-pNA
Sequence three letter code
  • Ac-Asp-Glu-Val-Asp-pNA
CAS registry number
  • 189950-66-1
Molecular Formula
  • C26H34N6O13
Molecular Mass/ Weight
  • 638.6
Properties
Absorbance (nm)
  • 405
Modification
Conjugation type
  • Conjugates
Modification Name
Conjugation
  • Conjugated
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Storage Conditions
  • - 20 °C Protected from light
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Large-scale expression in Escherichia coli and efficient purification of precursor and active caspase-7 by introduction of thrombin cleavage sites

Protein Express Purification . 2009 Sep 29 ; 69(1) 29 | DOI : 10.1016/j.pep.2009.09.014

  • Y. Lee

Induction of differentiation rescues HL-60 cells from Rana catesbeiana ribonuclease-induced cell death.

FEBS Lett . 2002 Nov 20 ; 531(3) 421 | DOI : 10.1016/s0014-5793(02)03577-9

  • C-W. Wei

Synergism of Rana catesbeiana ribonuclease and IFN-gamma triggers distinct death machineries in different human cancer cells.

FEBS Lett . . 2005 Jan 03 ; 579(1) 265 | DOI : 10.1016/j.febslet.2004.11.086

  • C-HA. Tang

Synergism of Rana catesbeiana ribonuclease and IFN-γ triggers distinct death machineries in different human cancer cells.

FEBS Lett . 2005 Jan 03 ; 579(1) 265 | DOI : 10.1016/j.febslet.2004.11.086

  • C-HA. Tang

Arsenic and fluoride induce neural progenitor cell apoptosis.

Toxicol Lett . 2011 Mar 23 ; 203(3) 237 | DOI : 10.1016/j.toxlet.2011.03.023.

  • R. Rocha

References

Substrate specificities of caspase family proteases.

J Biol Chem . 1997 Apr 01 ; 272(15) 9677 | DOI : https://doi.org/10.1074/jbc.272.15.9677

  • RV. Talanian
  • et al

Activation of the CPP32 protease in apoptosis induced by 1-ß-D-arabinofuranosylcytosine and other DNA-damaging agents

Blood . 1996 Sep 15 ; 88(6) 1936 | DOI : https://doi.org/10.1182/blood.V88.6.1936.bloodjournal8861936

  • R. Datta
  • et al

Apoptosis and its clinical impact

Head Neck . 2001 May 01 ; 23(5) 409 | DOI : https://doi.org/10.1002/hed.1052

  • BR. Gastman
  • et al

Caspase 8: an efficient method for large-scale autoactivation of recombinant procaspase 8 by matrix adsorption and characterization

Protein Expr Purif . 2002 May 25 ; 18(3) 378 | DOI : https://doi.org/10.1006/prep.2000.1205

  • KA. Koeplinger
  • et al

Caspases: enemies within.

Science . 1998 Aug 28 ; 281(5381 1312 | DOI : 10.1126/science.281.5381.1312

  • NA Thornberry
  • Y. Lazebnik

Caspases: key players in programmed cell death

Curr Opin Struct Biol . 2000 Dec 01 ; 10(6) 649 | DOI : https://doi.org/10.1016/S0959-440X(00)00146-9

  • MG. Grutter
  • et al

Catalytic properties of the caspases

Cell Death Differ . 1999 Nov 24 ; 6 1054 | DOI : https://doi.org/10.1038/sj.cdd.4400599

  • HR. Stennicke
  • GS. Salvesen

Enzymatic activity of two caspases related to interleukin-1beta-converting enzyme.

Eur J Biochem . 1998 Apr 01 ; 253(1) 76 | DOI : https://doi.org/10.1046/j.1432-1327.1998.2530076.x

  • F. Fassy
  • et al

Properties of the caspases

Biochim Biophys Acta . 1998 Nov 24 ; 1387(1-2) 17 | DOI : https://doi.org/10.1016/S0167-4838(98)00133-2

  • HR. Stennicke
  • GS. Salvesen