SensoLyte® Homogeneous AFC Caspase-3/7 Assay Kit Fluorimetric - 1 kit
- Cat.Number : AS-71114
- Manufacturer Ref. :
-
Availability :
In stock
- Shipping conditions : Ice fees will apply
Alternative choices
Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).
The SensoLyte® Homogeneous AFC Capase-3/7 Assay Kit uses Ac-DEVD-AFC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AFC generates the AFC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=380 nm/500 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.
Specifications
Packaging | |
Kits components |
|
---|---|
Properties | |
Absorbance (nm) |
|
Emission (nm) |
|
Storage & stability | |
Storage Conditions |
|
Activity | |
Application | |
Biomarker Target | |
Detection Method | |
Research Area | |
Sub-category Research Area | |
Usage |
|
Downloads
You may also be interested in the following product(s)
Citations
Inhibition of vascular calcification by block of intermediate conductance calcium-activated potassium channels with TRAM-34
Pharmacol Res . 2014 May 06 ; 85 6 | DOI : 10.1016/j.phrs.2014.04.013
- C. Freise
Caspase-like activity during aging and cell death in the toxic dinoflagellate< i> Karenia brevis
Harmful Algae . 2013 Oct 20 ; 31 41 | DOI : 10.1016/j.hal.2013.08.005
- J.G. Johnson
Glomerular cell death and inflammation with high-protein diet and diabetes
Nephrol Dial Transplant. . 2013 Jan 12 ; 28(7) 1711 | DOI : 10.1093/ndt/gfs579
- R.L. Meek
The inhibitor of Ca(2+)-dependent K+ channels TRAM-34 blocks growth of hepatocellular carcinoma cells via downregulation of estrogen receptor alpha mRNA and nuclear factor-kappaB
Invest New Drugs . 2012 Oct 02 ; 31(2) 452 | DOI : 10.1007/s10637-012-9879-6
- C. Freise
A hepatoprotective Lindera obtusiloba extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines
BMC Complement Altern Med . 2011 May 12 ; 11 39 | DOI : 10.1186/1472-6882-11-39
- C. Freise
An evolved ribosome-inactivating protein targets and kills human melanoma cells in vitro and in vivo
Mol Cancer . 2010 Feb 03 ; 9 28 | DOI : 10.1186/1476-4598-9-28
- M. Cheung
An active extract of Lindera obtusiloba inhibits adipogenesis via sustained Wnt signaling and exerts anti-inflammatory effects in the 3T3-L1 preadipocytes
J Nutr Biochem . 2010 Jan 25 ; 21(12) 1170 | DOI : 10.1016/j.jnutbio.2009.09.013
- C. Freise
Extracts of Lindera obtusiloba induce antifibrotic effects in hepatic stellate cells via suppression of a TGF-β-mediated profibrotic gene expression pattern
J Nutr Biochem . 2008 Sep 27 ; 20(8) 597 | DOI : 10.1016/j.jnutbio.2008.06.003
- M. Rueh
Synergistic inhibition of tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis in human pancreatic β cells by Bcl-2 and X-linked inhibitor of apoptosis
Hum Immunol . 2005 Mar 01 ; 66(3) 274 | DOI : 10.1016/j.humimm.2004.12.002
- D. Ou