IMG-EN-AS-25273-5-01.jpg
Peptides

Caspase 3 (Apopain) Substrate 1f, fluorogenic - 5 mg

$221.00
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  • Cat.Number : AS-25273-5
  • Availability :
    In production

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Caspase-3 substrate with kcat/Km = 1.3 ×106 M-1s-1, Caspase-7 substrate with kcat/Km = 1.3 ×104 M-1s-1;
AFC (7-amino-4-trifluoromethylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AFC peptides by caspases generate strongly fluorescent AFC that is monitored fluorimetrically at 500-510 nm with excitation of 370-390 nm.

Specifications

Chemistry
Sequence one letter code
  • Ac-DEVD-AFC
Sequence three letter code
  • Ac-Asp-Glu-Val-Asp-AFC
CAS registry number
  • 201608-14-2
Molecular Formula
  • C30H34F3N5O13
Molecular Mass/ Weight
  • 729.7
Properties
Absorbance (nm)
  • 380
Emission (nm)
  • 500
Modification
Conjugation type
  • Fluorescent dyes
Modification Name
Conjugation
  • Conjugated
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Storage Conditions
  • - 20 °C Protected from light
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Large-scale expression in Escherichia coli and efficient purification of precursor and active caspase-7 by introduction of thrombin cleavage sites.

Protein Express Purification . 2009 Sep 25 ; 69(1) 29 | DOI : 10.1016/j.pep.2009.09.014

  • Y. Lee

References

Activation of Multiple Interleukin-1β Converting Enzyme Homologues in Cytosol and Nuclei of HL-60 Cells during Etoposide-induced Apoptosis

J Biol Chem . 1997 Mar 01 ; 272(11) 7421 | DOI : https://doi.org/10.1074/jbc.272.11.7421

  • LM. Martins
  • et al

BAX-induced cell death may not require interleukin 1β-converting enzyme-like proteases

Proc Natl Acad Sci USA . 1996 Dec 10 ; 93(25) 14559 | DOI : https://doi.org/10.1073/pnas.93.25.14559

  • J. Xiang
  • et al

Fluorometric and colorimetric detection of caspase activity associated with apoptosis

Anal Biochem . 1997 Aug 15 ; 251(1) 98 | DOI : https://doi.org/10.1006/abio.1997.2220

  • V. Gurtu
  • et al

X-linked IAP is a direct inhibitor of cell-death proteases

Nature . 1997 Jul 17 ; 388 300 | DOI : https://doi.org/10.1038/40901

  • QL. Deveraux
  • et al