IMG-EN-AS-85102-01.jpg
Labeling-detection

Collagen (Type I), FITC conjugated Water Insoluble - 5 mg

$131.00
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  • Cat.Number : AS-85102
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Together with the water-soluble collagen (Type I) conjugate (Cat# AS-85111), this water-insoluble fluorescent collagen (type I) can be used for fluorometric measurement of collagenase activity. It is heavily labeled with FITC, resulting in almost total quenching of the conjugates fluorescence. Protease-catalyzed hydrolysis slowly yields brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. Although this fluorescent collagen is more slowly digested by MMP-1 than water-soluble collagen (Cat# AS-85111), it is more specific for the enzyme.

Specifications

Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Color
Quantity & Purity
Purity
  • Minimum Purity: 95%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • It is insoluble in water, aqueous buffers, and organic solvents and may be mechanically minced when preparing it for enzymatic reaction
Storage Conditions
  • Store at -20°C and protect from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • Bovine

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References

A handy assay for collagenase using reconstituted fluorescein-labeled collagen fibrils

Anal Biochem . 1980 Nov 01 ; 108(2) 230 | DOI : https://doi.org/10.1016/0003-2697(80)90574-6

  • A. Baici
  • et al

Enzymatic degradation of collagen-guided tissue regeneration membranes by periodontal bacteria.

Clin Oral Implants Res . 2003 May 20 ; 14(3) 263 | DOI : https://doi.org/10.1034/j.1600-0501.2003.140302.x

  • MN. Sela
  • et al