Labeling-detection

Collagen (Type I), FITC conjugated Water Insoluble - 5 mg

$104.00
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  • Cat.Number : AS-85102
  • Availability :
    In stock
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Quantity

Together with the water-soluble collagen (Type I) conjugate (Cat# AS-85111), this water-insoluble fluorescent collagen (type I) can be used for fluorometric measurement of collagenase activity. It is heavily labeled with FITC, resulting in almost total quenching of the conjugates fluorescence. Protease-catalyzed hydrolysis slowly yields brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. Although this fluorescent collagen is more slowly digested by MMP-1 than water-soluble collagen (Cat# AS-85111), it is more specific for the enzyme.

Specifications

Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Color
Quantity & Purity
Purity
  • Minimum Purity: 95%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • It is insoluble in water, aqueous buffers, and organic solvents and may be mechanically minced when preparing it for enzymatic reaction
Storage Conditions
  • -20°C desiccated and protected from light
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • Bovine

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