IMG-EN-AS-85111-01.jpg
Labeling-detection

Collagen (Type I), FITC conjugated Water soluble - 1 mg

$336.00
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  • Cat.Number : AS-85111
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This product is water soluble and is used for quick fluorometric measurement of collagenase activity. In this protease substrate, collagen is heavily labeled with FITC, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. This fluoresceinated collagen is useful for detecting MMP-1 activity.

Specifications

Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Color
Quantity & Purity
Concentration
  • 1 mg/ml
Storage & stability
Form
  • In solution
Resuspension condition
  • Water
Storage Buffer
  • 0.01 M acetic acid
Storage Conditions
  • Store at -20°C and protect from light.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • Bovine

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References

A handy assay for collagenase using reconstituted fluorescein-labeled collagen fibrils

Anal Biochem . 1980 Nov 01 ; 108(2) 230 | DOI : https://doi.org/10.1016/0003-2697(80)90574-6

  • A. Baici
  • et al

A simple and rapid assay for collagenase activity using fluorescence-labeled substrate

Kurume Med . 1980 Jan 01 ; 27(2) 63 | DOI : 10.2739/kurumemedj.27.63

  • K. Sugiyama
  • et al

Enzymatic degradation of collagen-guided tissue regeneration membranes by periodontal bacteria.

Clin Oral Implants Res . 2003 May 20 ; 14(3) 263 | DOI : https://doi.org/10.1034/j.1600-0501.2003.140302.x

  • MN. Sela
  • et al