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Assay-kits

SensoLyte® Plus 520 MMP-1 Assay Kit Fluorimetric and Enhanced Selectivity - 1 kit

$663.00
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  • Cat.Number : AS-72012
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    In stock
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The SensoLyte® Plus 520 MMP-1 Assay Kit is designed for specifically detecting MMP-1 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-1 monoclonal antibody is used in combination with an MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-1-induced cleavage of the FRET substrate.

Specifications

Packaging
Kits components
  • Component A: Microplate coated with monoclonal anti human MMP-1 antibody: 12 X 8 black strips Component B: MMP-1 standard, recombinant human proMMP-1: 10 µg/mL, 10 µL Component C: MMP dilution buffer: 10 mL Component D: 10 X Wash buffer: 50 mL Component E: APMA, 4-aminophenylmercuric acetate (Caution: Contains organic mercury. Dispose according to regulations): 100 mM, 150 µL Component F: MMP-1 substrate 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 50 µL Component G: Assay buffer: 50 mL Component H: Stop Solution: 10 mL Component I: Adhesive cover strip: 3 sheets
Chemistry
UniProt number
  • P03956
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component B at -80 °C. Store all other components at -20 °C. Components D, G, H and I can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.3 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

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Iron sensitizes keratinocytes and fibroblasts to UVA-mediated matrix metalloproteinase-1 through TNF-α and ERK activation

Exp Dermatol . 2011 Mar 01 ; 20 249 | DOI : 10.1111/j.1600-0625.2010.01152.x

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Proteolytic activity in wound fluids and tissues derived from chronic venous leg ulcers

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PLoS One . 2011 May 26 ; 6 e20348 | DOI : 10.1371/journal.pone.0020348

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Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth

J Biol Chem. . 2014 Mar 20 ; 289(19) 13295 | DOI : 10.1074/jbc.M114.550541

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Mechanisms of GLP-1 receptor–independent renoprotective effects of the dipeptidyl peptidase type 4 inhibitor linagliptin in GLP-1 receptor knockout mice with 5/6 nephrectomy

Kidney International . 2019 Jun 01 ; 95(6) 1373 | DOI : https://doi.org/10.1016/j.kint.2019.01.010

  • A. Hasan
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Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis.

Cell Res. . 2020 Jun 16 ; 30 794 | DOI : https://doi.org/10.1038/s41422-020-0354-1

  • J. Wu
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References

Iron sensitizes keratinocytes and fibroblasts to UVA-mediated matrix metalloproteinase-1 through TNF-α and ERK activation

Exper Dermatol . 2011 Mar 01 ; 20(3) 249 | DOI : https://doi.org/10.1111/j.1600-0625.2010.01152.x

  • J. Jian
  • et al