IMG-EN-AS-29971-5-01.jpg
Peptides

EGF Receptor Substrate 2 [DADE-pY-LIPQQG], 5-FAM labeled - 5 mg

$1,460.00
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  • Cat.Number : AS-29971-5
  • Availability :
    In production

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The labeled phosphopeptide is an excellent substrate for mammalian protein tyrosine phosphatase 1B (Km = 3.9 µM) and Yersinia PTP. The sequence is derived from an autophosphorylation site (Tyr992) of EGFR. The labeled peptide is used to assay PTB based on marked fluorescence increase upon the removal of the phosphate group (Em = 305 nm and Ex = 282 nm)

Specifications

Chemistry
Sequence one letter code
  • 5-FAM-DADE-pY-LIPQQG
Sequence three letter code
  • 5-FAM-Asp-Ala-Asp-Glu-pTyr-Leu-Ile-Pro-Gln-Gln-Gly-OH
Molecular Formula
  • C76H93N12O30P
Molecular Mass/ Weight
  • 1686.7
Properties
Absorbance (nm)
  • 282
Emission (nm)
  • 305
Modification
Conjugation type
  • Fluorescent dyes
Modification Name
Conjugation
  • Conjugated
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Storage Conditions
  • - 20 °C Protected from light
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • Mammal

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References

A Continuous Spectrophotometric and Fluorometric Assay for Protein Tyrosine Phosphatase Using Phosphotyrosine-Containing Peptides

Anal Biochem . 1993 May 15 ; 211(1) 7 | DOI : https://doi.org/10.1006/abio.1993.1224

  • ZY. Zhang
  • et al

Molecular Basis for Substrate Specificity of Protein-tyrosine Phosphatase 1B

JBC . 1998 Oct 01 ; 273(41) 26368 | DOI : https://doi.org/10.1074/jbc.273.41.26368

  • M. Sarmiento
  • et al