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Peptides

Kinase Substrates Library, Group I, biotinylated, 180 distinct peptide mixtures - 1 Set

$6,806.00
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  • Cat.Number : AS-62017-1
  • Availability :
    In production

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This positional scanning synthetic peptide combinatorial library (PS-SPCL) has been used in rapid identification of serine/threonine kinase phosphorylation motif. The use of this Group I of 180 distinct peptide mixtures, in combination with Group II of 18 distinct peptide mixtures (cat# 62335), can provide sequence preference information of the different kinases. Amount provided is 1 mg of peptide mixture x 180 vials.
Sequences:
Y-A-Z-X-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH2
Y-A-X-Z-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH2
Y-A-X-X-Z-X-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH2
Y-A-X-X-X-Z-X-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH2
Y-A-X-X-X-X-Z-S/T-X-X-X-X-A-G-K-K(LC-Biotin)-NH2
Y-A-X-X-X-X-X-S/T-Z-X-X-X-A-G-K-K(LC-Biotin)-NH2
Y-A-X-X-X-X-X-S/T-X-Z-X-X-A-G-K-K(LC-Biotin)-NH2
Y-A-X-X-X-X-X-S/T-X-X-Z-X-A-G-K-K(LC-Biotin)-NH2
Y-A-X-X-X-X-X-S/T-X-X-X-Z-A-G-K-K(LC-Biotin)-NH2
X = degenerate mixture of the 17 natural amino acids excluding cysteine, serine, and threonine. LC = 'long chain' version with an additional aminohexanoic acid spacer between the biotin and lysine side chain. S/T = equimolar mixture of serine and threonine. Z = fixed position varied between the 20 natural amino acids.

Specifications

Modification
Conjugation
  • Unconjugated
Quantity & Purity
Purity
  • Unpurified
Storage & stability
Form
  • Lyophilized
Storage Conditions
  • - 20 °C
Activity
Application
Biomarker Target
Research Area
Sub-category Research Area
Usage
  • Research use

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References

A rapid method for determining protein kinase phosphorylation specificity

Nature Methods . 2004 Sep 29 ; 173(1) 27 | DOI : https://doi.org/10.1038/nmeth708

  • JE. Hutti
  • et al

Determining protein kinase substrate specificity by parallel solution-phase assay of large numbers of peptide substrates

Nature Protocols . 2006 Jun 27 ; 1 375 | DOI : https://doi.org/10.1038/nprot.2006.57

  • BB. Turk
  • et al