Kinase Substrates Library, Group II, biotinylated, 18 distinct peptide mixtures - 1 Set
- Cat.Number : AS-62335
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This positional scanning synthetic peptide combinatorial library (PS-SPCL) has been used in rapid identification of serine/threonine kinase phosphorylation motif. The use of this Group II of distinct 18 peptide mixtures, in combination with Group I of 180 distinct peptide mixtures (cat# 62017-1), can provide sequence preference information of different kinases. Amount provided is 1 mg of peptide mixture x 18 vials.
Sequences:
Y-A-pT-X-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-pT-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-pT-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-pT-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-pT-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-pT-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-X-pT-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-X-X-pT-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-X-X-X-pT-A-G-K-K(LC-biotin)-NH2
Y-A-pY-X-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-pY-X-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-pY-X-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-pY-X-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-pY-S/T-X-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-pY-X-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-X-pY-X-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-X-X-pY-X-A-G-K-K(LC-biotin)-NH2
Y-A-X-X-X-X-X-S/T-X-X-X-pY-A-G-K-K(LC-biotin)-NH2
X = degenerate mixture of the 17 natural amino acids excluding cysteine, serine, and threonine. LC = 'long chain' version with an additional aminohexanoic acid spacer between the biotin and lysine side chain. S/T = equimolar mixture of serine and threonine. pT is phosphothreonine, and pY is phosphotyrosine.
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References
A rapid method for determining protein kinase phosphorylation specificity
Nature Methods . 2004 Sep 29 ; 173(1) 27 | DOI : https://doi.org/10.1038/nmeth708
- JE. Hutti
- et al
Determining protein kinase substrate specificity by parallel solution-phase assay of large numbers of peptide substrates
Nature Protocols . 2006 Jun 27 ; 1 375 | DOI : https://doi.org/10.1038/nprot.2006.57
- BB. Turk
- et al