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Labeling-detection

FDG [Fluorescein di-ß-D-galactopyranoside] - 5 mg

$190.00
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  • Cat.Number : AS-85600
  • Availability :
    In production
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Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive fluorogenic substrates available for detecting β-galactosidase. The colorless and nonfluorescent FDG is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties (Ex/Em=492/520 nm) that match the optimal detection window of most fluorescence instruments. Galactosidase-catalyzed hydrolysis of FDG can be followed by fluorescence increase around 520 nm. Alternatively, FDG can also be used to detect β-galactosidase in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). In general, fluorescence-based measurements can be several orders of magnitude more sensitive than absorption-based measurements. FDG has been widely used for identifying lacZ-positive cells with fluorescence microscopy and flow cytometry. FDG is also used to detect β-galactosidase expression in live cells. Fluorescence-based assays employing FDG are also reported to be 100 to 1000-fold more sensitive than radioisotope-based ELISAs.

Specifications

Chemistry
CAS registry number
  • 17817-20-8
Molecular Formula
  • C32H32O15
Molecular Mass/ Weight
  • 656.6
Properties
Absorbance (nm)
  • 492
Emission (nm)
  • 520
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMSO
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Common Origins of Hippocampal Ivy and Nitric Oxide Synthase Expressing Neurogliaform Cells.

J Neurosci . 2010 Feb 10 ; 30(6) 2165 | DOI : 10.1523/JNEUROSCI.5123-09.2010.

  • L. Tricoire

References

A fluorimetric method using fluorescein di-β-d-galactopyranoside for quantifying the senescence-associated β-galactosidase activity in human foreskin fibroblast Hs68 cells.

Anal Biochem . 2004 Feb 15 ; 325(2) 337 | DOI : https://doi.org/10.1016/j.ab.2003.11.012

  • NC. Yang
  • ML. Hu

Detection of β-lactamase reporter gene expression by flow cytometry

Cytometry . 2003 Feb 01 ; 51A(2) 68 | DOI : https://doi.org/10.1002/cyto.a.10018

  • T. Knapp
  • et al

Flow cytometric study of differentiating cultures of Bacillus subtilis

Cytometry . 1995 Aug 01 ; 20(4) 324 | DOI : https://doi.org/10.1002/cyto.990200408

  • JD. Chung
  • et al

Involvement of macrophage mannose receptor in the binding and transmission of HIV by macrophages

Eur J Immunol . 2003 Feb 01 ; 33(2) 483 | DOI : https://doi.org/10.1002/immu.200310024

  • DG. Nguyen
  • JE. Hildreth

Verapamil inhibition of enzymatic product efflux leads to improved detection of β-galactosidase activity in lacZ-transfected cells

Cytometry . 1997 May 01 ; 28(1) 36 | DOI : https://doi.org/10.1002/(SICI)1097-0320(19970501)28:1<36::AID-CYTO4>3.0.CO;2-F

  • M. Poot
  • S. Arttamanglul

Reliable Transient Promoter Assay Using Fluorescein-di-β-D-galactopyranoside Substrate

DNA Cell Biol . 2009 Mar 25 ; 9(4) 279 | DOI : https://doi.org/10.1089/dna.1990.9.279

  • K. Ikenaka
  • et al