Labeling-detection

HiLyte™ Fluor 488 hydroxylamine, HCl salt single isomer - 1 mg

$359.00
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  • Cat.Number : AS-64348
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

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Quantity

HiLyte™ Fluor 488 hydroxylamine is a carbonyl-reactive labeling dye, which reacts more readily with aldehydes at physiological pH than other primary amine-containing reagents (such as hydrazides and amines). The reaction of HiLyte™ Fluor 488 hydroxylamine with aldehydes yields oxime ethers, which are stable at physiological conditions. Therefore, HiLyte™ Fluor 488 hydroxylamine can be used to directly detect aldehydes in proteins and DNA. Extinction Coefficient (M-1cm-1): 70,000 Fluorescence quantum yield: 0.91 Fluorescence Life Time (ns): 4.1

Specifications

Chemistry
Molecular Mass/ Weight
  • 525.9
Properties
Absorbance (nm)
  • 498
Emission (nm)
  • 525
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥90%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO: Prepare 10 mg/ml dye stock solution in high quality anhydrous DMSO. Completely dissolve all dye content by vortexing. Dye solution should be prepared fresh, immediately before an experiment. Long-term storage in solution form may reduce dye activity. Any solution containing the dye should be protected from light.
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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References

A Flexible Method for the Conjugation of Aminooxy Ligands to Preformed Complexes of Nucleic Acids and Lipids

ChemmedChem . 2008 Sep 15 ; 3(9) 1356 | DOI : https://doi.org/10.1002/cmdc.200800084

  • JG. Hecker

High-efficiency labeling of sialylated glycoproteins on living cells

Nature methods . 2009 Mar 01 ; 6(3) 207 | DOI : 10.1038/nmeth.1305

  • Y. Zeng
  • et al

Synthesis of fluorescent probes for the detection of abasic sites in DNA

Tetrahedron Lett. . 1997 Apr 14 ; 53(15) 5485 | DOI : https://doi.org/10.1016/S0040-4020(97)00235-4

  • D. Boturyn
  • et al