The 10~12 kDa aspartic protease of human immunodeficiency virus-1 (HIV-1), active only when it forms a homodimer, is required for the post-translational cleavage of the precursor polyproteins, Gag, Gag-Pol, Pol, and Nef. Since the cleavages of precursors are essential for the maturation of HIV infectious particles, this protease is one of the key targets for developing anti-AIDS drugs
The recombinant HIV-1 protease (wild type, NCBI accession: NC_001802) contains the 99 amino acid sequence as reported by Schneider, et al. (Cell 54, 363;1988) with 6x His-tag at its C-terminal. 100-200 ng of HIV-1 protease is sufficient for FRET-based activity assays
Molecular Mass/ Weight
Quantity & Purity
≥95% by SDS-PAGE
Storage & stability
Store at -80°C. Avoid repeated freeze-thaw cycles.