Protein

Human MMP-14 - 100 µL

  • Cat.Number : AS-72068
  • Availability :
    In stock
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-14 (MT1-MMP), membrane-type MMP, plays an important role in tumor invasion. MMP-14 is expressed on the surface of invasive tumor cells, in stromal cells of human colon, breast, and head and neck carcinomas. MMP-14 is secreted as zymogen with a prodomain, a catalytic domain, a hinge region, a hemopexin-like domain, and a transmembrane domain. It can activate pro-MMP-23 and degrade a variety of substrates, including fibrillar collagens I, II, III, fibronectin, vitronectin and laminin-1.

A truncated human MMP-14 with His-tag was expressed in E. coli. The Mr on SDS-PAGE is 31-kDa. Incubation with 1 mM APMA at 37°C for 2 hr will activate MMP-14. Its activity can be measured by FRET peptides

Specifications

Chemistry
UniProt number
  • P50281
Molecular Mass/ Weight
  • 31 kDa
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • 10 µg/ml
Purity
  • >95%
Storage & stability
Form
  • In solution
Storage Buffer
  • 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM CaCl2, 1 mg/mL BSA.
Storage Conditions
  • Store at -80 ºC. Avoid repeated freeze-thaw cycles.
Activity
Application
Biomarker Target
Unit definition
  • One unit of protease hydrolyzes 1 picomole of Mca-Pro-Leu-Gly-LeuDap(Dnp)-Ala-Arg-NH2 per minute at pH 7.5 at 25°C.
Specificity
  • Human MMP-14
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • human

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