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Protein

Human MMP-2 - 100 µL

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  • Cat.Number : AS-72005
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    In stock
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-2 (72-kDa gelatinase-A) is involved in tumor development and metastasis. It is proposed as a therapeutic target for cancer.

Recombinant human MMP-2 was expressed as a pro-enzyme from its DNA sequence, transfected into CHO cells. The pro-MMP-2 can be fully activated by incubating with 1 mM APMA at 37°C for 20 min to 1 hr. Its activity can be measured in FRET-based enzymatic assays. 10-20 ng of enzyme is sufficient for FRET-based assay.

Specifications

Chemistry
UniProt number
  • P08253
Molecular Mass/ Weight
  • 72 kDa
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • 10 µg/ml
Purity
  • ≥90% by SDS-PAGE
Storage & stability
Form
  • In solution
Storage Buffer
  • 50mM Tris-HCl at pH 7.5, 0.2M NaCl, 5mM CaCl2, 1uM ZnCl2 , 0.05% Brij35 and 1mg/ml BSA.
Storage Conditions
  • Store at -80°C. Avoid repeated freeze-thaw cycles.
Activity
Application
Biomarker Target
Unit definition
  • One unit of protease hydrolyzes 1 picomole of Mca-Pro-Leu-Gly-LeuDap(Dnp)-Ala-Arg-NH2 per minute at pH 7.4 at 25° C
Specificity
  • Human MMP-2
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • human

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