Protein

Human MMP-3 - 100 µL

  • Cat.Number : AS-72006
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    In stock
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known. MMP-3 (stromelysin-1, transin-1) has been shown to involved in tumor metastasis3 and rheumatoid arthritis. Therefore it is proposed as a therapeutic target for these diseases. The native pro-MMP-3 is Mr 59/57-kDa doublet, which can be autocatalyzed to an active form of 45-kDa, and is then processed partially to a second active form of 28-kDa

Human MMP-3 catalytic domain was expressed as 253 amino acid sequence (Pro19-Glu271) in E. coli. The molecular mass is approximately 28/29 kDa on SDS-PAGE. Recombinant human MMP-3 enzyme has catalytic domain only and doesn’t need APMA activation before enzyme assay. Its activity can be measured in FRET-based enzymatic assays

Specifications

Chemistry
UniProt number
  • P08254
Molecular Mass/ Weight
  • 28 kDa
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • 10 µg/ml
Storage & stability
Form
  • In solution
Storage Buffer
  • 50mM HEPES, pH 7.5, 0.05% Brij 35, 10 mM CaCl2, 1 mg/mL BSA, 2mM sodium azide.
Storage Conditions
  • Store at -80 ºC. Avoid repeated freeze-thaw cycles.
Activity
Application
Biomarker Target
Unit definition
  • One unit of protease hydrolyzes 1 picomole of Mca-Arg-Pro-Lys-Pro-Val-Glu Nva-Trp-Arg-Lys(Dnp)-NH2 per minute at pH 7.5 at 25°C.
Specificity
  • Human MMP-3
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • human

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