Protein

Human MMP-3 - 100 µL

$103.00
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  • Cat.Number : AS-72006
  • Availability :
    In stock
  • Shipping conditions : Ice fees will apply

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Quantity

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known. MMP-3 (stromelysin-1, transin-1) has been shown to involved in tumor metastasis3 and rheumatoid arthritis. Therefore it is proposed as a therapeutic target for these diseases. The native pro-MMP-3 is Mr 59/57-kDa doublet, which can be autocatalyzed to an active form of 45-kDa, and is then processed partially to a second active form of 28-kDa.

Recombinant human MMP-3 enzyme was expressed as catalytic domain in E. coli. The molecular mass is 19.5 kDa. Purity is >95% by SDS-PAGE. Recombinant human MMP-3 catalytic domain doesn’t need APMA activation before enzyme assay. Its activity can be measured in FRET-based enzymatic assays.

Specifications

Chemistry
UniProt number
  • P08254
Molecular Mass/ Weight
  • 19.5 kDa
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • 10 µg/ml
Purity
  • ≥95% by SDS-PAGE
Storage & stability
Form
  • In solution
Storage Buffer
  • 50mM HEPES, pH 7.5, 0.05% Brij 35, 10 mM CaCl2, 1 mg/mL BSA, 2mM sodium azide.
Storage Conditions
  • Store at -80°C. Avoid repeated freeze-thaw cycles.
Activity
Application
Biomarker Target
Unit definition
  • One unit of protease hydrolyzes 1 picomole of Mca-Arg-Pro-Lys-Pro-Val-Glu Nva-Trp-Arg-Lys(Dnp)-NH2 (AnaSpec Cat#AS-27114) per minute at pH 7.5 at 25°C.
Specificity
  • Human MMP-3
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • human

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