Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known. MMP-7 (matrilysin, PUMP-1) is proposed as a potential anti-cancer drug target.
Recombinant human MMP-7 was expressed in E.coli. The molecular mass of zymogen is approximately 28-kDa on SDS-PAGE and the active form is 19-kDa. pro-MMP-7 can be fully activated by incubating with 1 mM APMA at 37°C for 1 hr. Its activity can be measured by FRET peptides. 5-10 ng of enzyme is sufficient for FRET-based assay.