Peptides

MBP, MAPK Substrate [APRTPGGRR], Biotinylated - 1 mg

  • Cat.Number : AS-60747
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This is an N-terminally biotinylated peptide. The sequence APRTPGGRR contains a native sequence derived from bovine myelin basic protein amino acids 95-98 (PRTP). The rest of the sequence is not derived from a native sequence, but is a synthetic construct. This substrate is specific for MAP kinases: p44MAPK [extracellular signal-regulated kinase 1 (ERK1)] and p42MAPK (ERK2). It contains the consensus sequence Pro-X-(Ser/Thr)-Pro that is recognized by MAP kinase. This peptide is the most efficient substrate for phosphorylation reaction by ERK. The substrate is phosphorylated by kinases on threonine 97 and can also be phosphorylated by MAPK p38.

Specifications

Chemistry
Sequence one letter code
  • Biotin-APRTPGGRR
Sequence three letter code
  • Biotin-Ala-Pro-Arg-Thr-Pro-Gly-Gly-Arg-Arg-OH
Molecular Formula
  • C49H84N20O13S
Molecular Mass/ Weight
  • 1193.4
Modification
Conjugation type
  • Biotins
Modification Name
Conjugation
  • Conjugated
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Storage Conditions
  • - 20 °C
Activity
Application
Biomarker Target
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • Synthetic construct

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References

A New Peptide Conjugate as a Highly Specific Substrate for MAP Kinase

J Biochem (Tokyo) . 1997 Jul 01 ; 122(1) 168 | DOI : 10.1093/oxfordjournals.jbchem.a021723

  • I. Kameshita
  • et al

Activation and Phosphorylation on Thr-160 of Nuclear-Targeted CDK2 Is ERK Dependent

Ann N. Y. Acad Sci . 2002 Nov 01 ; 973(1) 265 | DOI : https://doi.org/10.1111/j.1749-6632.2002.tb04646.x

  • NH. Lents
  • et al