Myelin Oligodendrocyte Glycoprotein (MOG) is a member of the immunoglobulin superfamily and is expressed exclusively in the central nervous system (CNS). Although MOG protein constitutes only 0.01-0.05% of the CNS myelin proteins, it was demonstrated that MOG protein is a crucial autoantigen for multiple sclerosis in humans and experimental autoimmune encephalomyelitis (EAE) in rodents and monkeys
The sequence (Accession #CAE84068) corresponding to the extracellular domain of rat MOG along with a 6x His tag was expressed in E. coli. The recombinant rat MOG (R-rMOG) was purified from urea denatured bacterial lysate using immobilized metal affinity chromatography (IMAC). The molecular mass of the recombinant rat MOG is 14.2 kDa.
Molecular Mass/ Weight
Quantity & Purity
≥95% by SDS-PAGE
Storage & stability
rat MOG must be thoroughly mixed directly with Complete Freund’s Adjuvant (CFA). Do not dilute recombinant rat MOG with buffers that have pH greater than 4.5. Protein will precipitate at pH higher than 4.5
25 mM sodium acetate buffer (pH=4.0)
Store at -80°C. Avoid repeated freeze-thaw cycles.