Labeling-detection

Resorufin ß-D-Galactopyranoside - 25 mg

$119.00
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  • Cat.Number : AS-85617
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

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Unlike FDG that requires a two-step hydrolysis to generate maximum fluorescence, resorufin β-D-galactopyranoside requires only a single-step hydrolysis reaction to attain full fluorescence. This substrate is especially useful for sensitive enzyme measurements in ELISAs.

Specifications

Chemistry
CAS registry number
  • 95079-19-9
Molecular Formula
  • C18H17NO8
Molecular Mass/ Weight
  • 375.3
Properties
Absorbance (nm)
  • 573
Emission (nm)
  • 585
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMSO
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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References

A fluorimetric method using fluorescein di-β-d-galactopyranoside for quantifying the senescence-associated β-galactosidase activity in human foreskin fibroblast Hs68 cells.

Anal Biochem . 2004 Feb 15 ; 325(2) 337 | DOI : https://doi.org/10.1016/j.ab.2003.11.012

  • NC. Yang
  • ML. Hu

Detection of β-galactofuranosidase production by Penicillium and Aspergillus species using 4-nitrophenyl β-D-galactofuranoside

J Appl Bacterio . 1989 Apr 01 ; 66(4) 311 | DOI : https://doi.org/10.1111/j.1365-2672.1989.tb02484.x

  • MA. Cousin
  • et al

Enzymatic synthesis of p-nitrophenyl 4(5)-O-beta-D-galactosyl-alpha-maltopentaoside as a substrate for human alpha-amylases

Anal Biochem . 1992 Apr 01 ; 202(1) 61 | DOI : https://doi.org/10.1016/0003-2697(92)90206-M

  • T. Usui
  • et al

Hydrolase activities in brain of neurological mutants: cerebroside galactosidase, nitrophenyl galactoside hydrolase, nitrophenyl glucoside hydrolase and sulphatase

JNC . 1969 Mar 01 ; 16(3) 457 | DOI : 10.1111/j.1471-4159.1969.tb10386.x

  • DM, Bowen
  • NS. Radin

Hydrolytic activity of alpha-galactosidases against deoxy derivatives of p-nitrophenyl alpha-D-galactopyranoside

Carbohydr Res . 2000 Feb 11 ; 324(2) 107 | DOI : https://doi.org/10.1016/S0008-6215(99)00281-5

  • W. Hakamata
  • et al

In Situ Histochemical Detection of β-Galactosidase Activity in Lung: Assessment of X-Gal Reagent in Distinguishing lacZ Gene Expression and Endogenous β-Galactosidase Activity

Hum Gene Ther . 1997 Sep 01 ; 8(13) 1545 | DOI : https://doi.org/10.1089/hum.1997.8.13-1545

  • DJ. Weiss
  • et al

Use of beta-maltosides (p-nitrophenyl-beta-D-maltoside, 2-chloro-4- nitrophenyl-beta-D-maltoside and 4-methylumbelliferyl-beta-D-maltoside) as substrates for the assay of neutral alpha-glucosidase from human kidney and urine

Clin Chim Acta . 1996 Jan 31 ; 244(2) 145 | DOI : https://doi.org/10.1016/0009-8981(95)06189-4

  • IS. Lukomskaya
  • et al

Synthesis of 4-nitrophenyl b-D-fucofuranoside and b-D-fucofuranosyl-(1“3)-D-mannopyranose: modified substrates for studies on catalytic requirements of b-D-galactofuranosidase

Carbohydr Res . 2000 Jan 12 ; 323(1-4) 7 | DOI : Chiocconi, A. et al.

  • A. Chiocconi
  • et al