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Assay-kits

SensoLyte® 490 MMP-1 Assay Kit Fluorimetric - 1 kit

$804.00
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  • Cat.Number : AS-71128
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MMP-1 (interstitial collagenase, fibroblast collagenase) is an extracellular protease. This enzyme plays a significant role in some diseases and has been identified as a drug screening target.
The SensoLyte® 490 MMP-1 Assay Kit uses an optimized fluorogenic peptide substrate for continuous measurement of the enzyme activity, providing speed, convenience and accuracy in analyzing MMP-1 and screening MMP-1 inhibitors.
Members of the MMP family have poor substrate sequence specificity, making it difficult to use a peptide substrate alone to differentiate the activity of a particular MMP from other MMPs. If several MMPs are coexisting in your samples and you would like to specifically measure MMP-1 activity, please choose the SensoLyte® Plus MMP-1 Assay Kit, Cat# AS-72012.

Specifications

Packaging
Kits components
  • Component A: MMP-1 substrate EDANS/DabcylPlus™ FRET peptide Ex/Em=340 nm/490 nm: upon cleavage 270 µL Component B: EDANS, fluorescence reference standard (1 mM, 10 µL), Ex/Em=340 nm/490 nm: 1 mM, 10 µL Component C: APMA, 4-aminophenylmercuric acetate: 1 M, 100 µL Component D: Assay buffer: 60 mL Component E: Stop solution: 30 mL
Chemistry
UniProt number
  • P03956
Properties
Absorbance (nm)
  • 340
Emission (nm)
  • 490
Storage & stability
Storage Conditions
  • Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at 4 °C for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 36 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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