Assay-kits

SensoLyte® 520 Cathepsin B Assay Kit Fluorimetric - 1 kit

$504.00
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  • Cat.Number : AS-72164
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    In stock
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Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin B is a member of the cathepsin family consisting of 12 cysteine proteases with broad exo- and endopeptidase activity. Elevated levels of cathepsin B were detected in metastases and neurological disorders including Alzheimer's disease (AD). The SensoLyte® 520 Cathepsin B Activity Assay Kit provides a QXL™ 520/HiLyte™ Fluor 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the QXL™ 520/HiLyte™ Fluor 488 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

Specifications

Packaging
Kits components
  • Component A: QXL™520/HiLyte™ Fluor 488 Cathepsin B substrate, Ex/Em=490 nm/520 nm upon cleavage: 2 mM, 50 µL Component B: HiLyte™ Fluor 488, fluorescence reference standard, Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: Cathepsin B enzyme, human liver: 5 µL Component D: Assay Buffer: 20 mL Component E: Cathepsin B inhibitor Ac-LVK-CHO: 100 µM, 10 µL Component F: DTT: 1 M, 100 µL
Chemistry
UniProt number
  • P07858
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Identification of a broad-spectrum antiviral small molecule against severe acute respiratory syndrome coronavirus and Ebola, Hendra, and Nipah viruses by using a novel high-throughput screening assay

J Virol . 2014 Feb 05 ; 88(8) 4353 | DOI : 10.1128/JVI.03050-13

  • H.A. Elshabrawy
  • et al

Development of Purkinje cell degeneration in a knockin mouse model reveals lysosomal involvement in the pathogenesis of SCA6

Proc Natl Acad Sci USA . 2012 Oct 10 ; 109(43) 17693 | DOI : 10.1073/pnas.1212786109

  • T. Unno
  • et al