IMG-EN-AS-72019-01.jpg
Assay-kits

SensoLyte® Plus 520 MMP-13 Assay Kit Fluorimetric and Enhanced Selectivity - 1 kit

$663.00
Check your price
  • Cat.Number : AS-72019
  • Availability :
    In stock
  • Shipping conditions : Ice fees will apply

Alternative choices

Quantity

Add to wishlist Bulk Quote

The SensoLyte® Plus 520 MMP-13 Assay Kit is designed for specifically detecting MMP-13 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-13 monoclonal antibody is used in combination with a MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-13-induced cleavage of the FRET substrate.

Specifications

Packaging
Kits components
  • Component A: Microplate coated with monoclonal anti human MMP-13 antibody: 12 x 8 black strips Component B: MMP-13 standard, recombinant human pro-MMP-13: 10 µg/mL, 10 µL Component C: MMP dilution buffer: 10 mL Component D: 10X Wash buffer: 50 mL Component E: APMA, 4-aminophenylmercuric acetate (Caution: Contain organic mercury. Dispose it according to your local regulations): 100 mM, 150 µL Component F: MMP-13 substrate 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 50 µL Component G: Assay buffer: 50 mL Component H: Stop Solution: 10 mL Component I: Adhesive cover strip: 3 sheets
Chemistry
UniProt number
  • P45452
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component B at -80 °C. Store all other components at -20 °C. Components D, G, H and I can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 3 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host

Downloads

You may also be interested in the following product(s)

IMG-EN-AS-60431-1-01.jpg

ADAMTS-4/Aggrecanase FRET Substrate, WAAG-3R - 1 mg

Cat.Number : AS-60431-1
$296.00 Excl. Tax
IMG-EN-AS-72257-01.jpg

MMP-13 (Catalytic Domain), human recombinant - 1 µg

Cat.Number : AS-72257
$124.00 Excl. Tax

Citations

Activation of matrix metalloproteinases 2, 9, and 13 by activated protein C in human osteoarthritic cartilage chondrocytes

Arthritis Rheumatol . 2014 Jun 01 ; 66 1525 | DOI : 10.1002/art.38401

  • M.T. Jackson

AP-1 as a regulator of MMP-13 in the stromal cell of giant cell tumor of bone

Biochem Res Int . 2011 Feb 17 ; 2011 164197 | DOI : 10.1155/2011/164197

  • I. Mak

The enhancement of chondrogenic differentiation of human mesenchymal stem cells by enzymatically regulated RGD functionalities

Biomaterials . 2008 May 01 ; 29 2370 | DOI : 10.1016/j.biomaterials.2008.01.035

  • C. Salinas

A role for CITED2, a CBP/p300 interacting protein, in colon cancer cell invasion

FEBS Lett . 2007 Dec 03 ; 581 5904 | DOI : 10.1016/j.febslet.2007.11.072

  • L. Bai

IL-1β-induced matrix metalloproteinase-13 is activated by a disintegrin and metalloprotease-28-regulated proliferation of human osteoblast-like cells

Exp Cell Res . 2014 Mar 05 ; 323 165 | DOI : 10.1016/j.yexcr.2014.02.018

  • N. Ozeki

Collagenase expression and activity in the stromal cells from giant cell tumour of bone

Bone . 2009 Feb 12 ; 44 865 | DOI : 10.1016/j.bone.2009.01.393

  • R.W. Cowan

Proteolytic activity in wound fluids and tissues derived from chronic venous leg ulcers

Wound Repair Regen. . 2009 Nov 01 ; 17 832 | DOI : 10.1111/j.1524-475X.2009.00547.x

  • A. Moor

Mechanisms of GLP-1 receptor–independent renoprotective effects of the dipeptidyl peptidase type 4 inhibitor linagliptin in GLP-1 receptor knockout mice with 5/6 nephrectomy

Kidney International . 2019 Jun 01 ; 95(6) 1373 | DOI : https://doi.org/10.1016/j.kint.2019.01.010

  • A. Hasan
  • et al

Design and Synthesis of Water-Soluble and Potent MMP-13 Inhibitors with Activity in Human Osteosarcoma Cells

Int J Mol Sci . 2021 Sep 15 ; 22(18) 9976 | DOI : 10.3390/ijms22189976

  • JM. Zapico
  • et al

Nose to Spine: spheroids generated by human nasal chondrocytes for scaffold-free nucleus pulposus augmentation

Acta Biomat . 2021 Oct 01 ; 134 240 | DOI : https://doi.org/10.1016/j.actbio.2021.07.064

  • A. Gryadunova
  • et al

HIV-1 Tat promotes astrocytic release of CCL2 through MMP/PAR-1 signaling

Glia . 2019 Sep 01 ; 67(9) 1719 | DOI : 10.1002/glia.23642

  • PL. Bozzelli
  • et al

RECK suppresses interleukin-17/TRAF3IP2-mediated MMP-13 activation and human aortic smooth muscle cell migration and proliferation.

J Cell Physiol. . 2019 Dec 01 ; 234(12) 22242 | DOI : 10.1002/jcp.28792

  • S. Mummidi
  • et al

Wnt16 Signaling Is Required for IL-1β-Induced Matrix Metalloproteinase-13-Regulated Proliferation of Human Stem Cell-Derived Osteoblastic Cells

Int. J. Mol. Sci. . 2016 Feb 06 ; 17(2) 221 | DOI : https://doi.org/10.3390/ijms17020221

  • N. Ozeki
  • et al

Mechanical loading inhibits hypertrophy in chondrogenically differentiating hMSCs within a biomimetic hydrogel.

J Mater Chem B Mater Biol Med. . 2016 May 28 ; 4(20) 3562 | DOI : 10.1039/c6tb00006a

  • EA. Aisenbrey
  • S. Bryant

Secreted matrix metalloproteinase-14 is a predictor for antifibrotic effect of IC-2-engineered mesenchymal stem cell sheets on liver fibrosis in mice

Regen Ther . 2021 Aug 26 ; 18 292 | DOI : https://doi.org/10.1016/j.reth.2021.08.004

  • K. Fukushima
  • et al

References

CRISPR-Cas9 targeting of MMP13 in human chondrocytes leads to significantly reduced levels of the metalloproteinase and enhanced type II collagen accumulation.

Osteoarthrit Cartil . 2019 Jan 01 ; 27(1) 140 | DOI : https://doi.org/10.1016/j.joca.2018.09.001

  • C. Seidl
  • et al