Assay-kits

SensoLyte® Thiol Quantitation Assay Kit “Colorimetric” - 1 kit

$300.00
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  • Cat.Number : AS-72136
  • Availability :
    In stock
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Quantity

Thiols, compounds that contain sulfhydryl group, are powerful reducing agents capable of acting as antioxidants, enzyme cofactors, and neuromodulators. The SensoLyte® Thiol Quantitation Assay Kit utilizes the widely used Elmann’s reagent for colorimetric measurement of thiol concentrations. The sulfhydryl group of thiol reacts with Ellman’s Reagent [5, 5’-dithiobis (2-nitrobenzoic acid)] and generates 2-nitro-5-thiobenzoic acid (TNB) producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to thiol concentration.

Specifications

Packaging
Kits components
  • Component A: Thiol Detection Reagent: 1 mL Component B: Reduced Glutathione (GSH) Standard: 10 mM, 100 µL Component C Assay Buffer: 100 mL
Properties
Absorbance (nm)
  • 420
Storage & stability
Storage Conditions
  • Store all kit components at -20°C. Protect Component A from light and moisture. Component C can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 1.5 uM
Research Area
Sub-category Research Area
Usage
  • Research use

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