Assay-kits

SensoLyte® Total GSH assay Kit Colorimetric - 1 kit

$434.00
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  • Cat.Number : AS-72153
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    In stock
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Glutathione (GSH) is an important antioxidant molecule that functions as a cofactor for a variety of enzymes and is involved in various biological processes. Total GSH includes reduced GSH and oxidized GSH (GSSG). The concentrations of GSH and GSSG and their molar ratio can be used for indicating the oxidative stress status.
The SensoLyte® Total GSH Quantitation Assay Kit utilizes the widely used Elmann’s reagent and an optimized enzymatic recycling method for the quantification of total glutathione. Glutathione reductase converts oxidized glutathione to reduced glutathione. Reduced GSH reacts with Ellman’s Reagent [5, 5’-dithiobis (2-nitrobenzoic acid)] and generates 2-nitro-5-thiobenzoic acid (TNB) producing a yellow color that can be detected at absorbance 405-415 nm. The intensity of the color produced is proportional to GSH concentration. The kit also provides 5-sulfosalicylic acid for protein removal from biological samples.

Specifications

Packaging
Kits components
  • Component A: Thiol Detection Reagent:1 mL Component B: Reduced Glutathione Standard: 10 mM, 200 µL Component C: GSH Reductase: 150 µL Component D: NADPH: 60 µL Component E: Assay Buffer: 100 mL Component F: 5-Sulfosalicylic Acid (SSA): 5 g
Properties
Absorbance (nm)
  • 410
Storage & stability
Storage Conditions
  • Store Components A, B, and D at -20°C. Protect Component A from light and moisture. Store Components C and F at 4°C. Component E can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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