Alkaline Phosphatase Reporter Gene Assays
Secreted alkaline phosphatase (SEAP) is widely used as a reporter gene to analyze gene expression, the activity of promoters and transcriptional factors in cell culture or animals.
Our SensoLyte® Secreted Alkaline Phosphatase Reporter Gene Assays provide sensitive assays of placental alkaline phosphatase for both secreted and membrane-bound forms of the enzyme. Our two kits allow a detection in colorimetry (using pNPP; detection at 405 nm) or chemiluminescence. Both kits are based on a “mix and read” assay protocol that is compatible with high throughput screening.
More about Phophatases
Phophatases hydrolyze a variety of organic phosphate monoesters to alcohols and inorganic phosphate. This important class of enzymes is involved in cellular signal transduction, cell metabolism and other biological activities. Some phosphatases serve as enzyme markers. For example, PALP-1, the gene for human placental alkaline phosphate, has been used as a eukaryotic receptor gene that is superior to IacZ for lineage studies in murine retina. This gene has also been engineered to produce a secreted alkaline phosphatase (SEAP), allowing quantitation of gene expression without disrupting the cells. Some phosphatases (such as protein phosphatase) are identified as drug screening targets.