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Fluorescent Dyes  >  Reactive Fluorescent Dyes  >>  5-FAM-X, SE

Product Name 5 - FAM - X, SE
6 - (Fluorescein - 5 - carboxamido)hexanoic acid, succinimidyl ester; 5 - FAM - X, NHS ester
Size 5 mg
Catalog # AS-81009
US$ $99
Purity TLC: >=98%, HPLC: 97%
Description

5-FAM-X, SE contains a seven-atom aminohexanoyl spacer (known as X) between the FAM fluorophore and the succinimidyl ester. The X spacer separates the fluorophore from the biomolecule to which it is conjugated. The X spacer may potentially reduce the quenching that typically occurs upon conjugation. We recommend this fluorescein derivative as the preferred dye for preparing fluoresceinated proteins when the fluorescence quenching of the labeling dyes by proteins is a serious problem.

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References 1. Czapski GA, et al. Med Sci Monit 7, 606-9; (2001).
2. Cooper WC, et al. Biophys J 78, 1449-57; (2000).
Molecular Weight 586.55
Molecular Formula C31H26N2O10
Spectral Properties Abs/Em = 494/521 nm
Solvent System DMF or DMSO
Product Citations "Knowles, J. et al. (2009). The p75 neurotrophin receptor promotes amyloid-β(1-42)-induced neuritic dystrophy in vitro and in vivo. J Neurosci 29, 10627."
     
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