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Fluorescent Dyes  >  Enzyme Detection Reagents  >>  Caspase 3 (Apopain) Substrate 1f, fluorogenic

Product Name Caspase 3 (Apopain) Substrate 1f, fluorogenic
Size 5 mg
Catalog # AS-25273-5
US$ $186

AFC (7-amino-4-trifluoromethylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AFC peptides by caspases generate strongly fluorescent AFC that is monitored fluorimetrically at 500-510 nm with excitation of 370-390 nm.

Caspase-3 substrate with kcat/Km = 1.3 106 M-1s-1, Caspase-7 substrate with kcat/Km = 1.3 104 M-1s-1

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References Gurtu V, et al. (1997). Fluorometric and colorimetric detection of caspase activity associated with apoptosis. Anal Biochem 251, 98-102; Martins LM, et al. (1997). Activation of multiple interleukin-1beta converting enzyme homologues in cytosol and nuclei of HL-60 cells during etoposide-induced apoptosis. J Biol Chem 272, 7421-30; Deveraux QL, et al. (1997). X-linked IAP is a direct inhibitor of cell-death proteases. Nature 388, 300-4; Xiang J, et al. (1996). BAX-induced cell death may not require interleukin 1 ?-converting enzyme-like proteases. Proc Natl Acad Sci USA 93, 14559-63.
Molecular Weight 729.7
(One-Letter Code)
(Three-Letter Code)
Ac - Asp - Glu - Val - Asp - AFC
Product Citations Lee, Y. et al. (2010). Large-scale expression in Escherichia coli and efficient purification of precursor and active caspase-7 by introduction of thrombin cleavage sites. Protein Express Purification 69, 29.
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