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Fluorescent Dyes  >  Enzyme Detection Reagents  >>  Collagen (Type I), FITC conjugated *Water Insoluble*

Product Name Collagen (Type I), FITC conjugated *Water Insoluble*
Size 5 mg
Catalog # AS-85102
US$ $104

Besides water-soluble collagen (Type I) conjugate (Cat#85111), AnaSpec also offers water-insoluble fluoresceinated collagen (type I). It can also be used for fluorometric measurement of collagenase activity. Cat#85102 is the water-insolulbe collagen that is heavily labeled with FITC, resulting in almost total quenching of the conjugates fluorescence. Protease-catalyzed hydrolysis slowly yields brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. Although this fluoresceinated collagen is more slowly digested by MMP-1 than water-soluble collagen (Cat#85111), it is more specific for the enzyme.

Minimum Purity: 95%

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References Sela MN, et al. (2003). Enzymatic degradation of collagen-guided tissue regeneration membranes by periodontal bacteria. Clin Oral Implants Res 14, 263-8, Baici A, et al. (1980). A handy assay for collagenase using reconstituted fluorescein-labeled collagen fibrils. Anal Biochem 108, 230-2.
Molecular Weight N/D
Spectral Properties Abs/Em = 492/515 nm
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