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Assay Kits  >  Viral/Bacterial Protease Assay Kits - HIV, HCV, TEV, WNV, Sortase  >>  SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric*

Product Name SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric*
Size 1 kit
Catalog # 71146
US$ $881
Description

The SensoLyte® 620 HCV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte™ Fluor TR (fluorophore) and QXL™ 610 (quencher) for a continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor TR is quenched by QXL™ 610. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of HiLyte™ Fluor TR is recovered and can be continuously monitored at excitation/emission = 590 nm/620 nm. With near red emission wavelength, this HiLyte™ Fluor TR/QXL™ 610-based FRET peptide avoids the autofluorescence interference from most test compounds. This feature makes this FRET peptide an ideal substrate for screening HCV protease inhibitors since some of screening compounds have strong autofluorescence at shorter wavelength, making other FRET peptide substrates inappropriate. The assays are performed in a convenient 96-well or 384-well microplate format. The kit contains: • HiLyte™ Fluor TR/QXL™ 610-based FRET peptide substrate (Ex/Em=590/620 nm upon cleavage) • Assay buffer • Fluorescence reference standard for calibration • A detailed protocol Kit Size: 100 assays

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Poster
Product Citations Zhu, Zhaowen, et al. (2010). Biliverdin inhibits hepatitis C virus NS3/4A protease activity: Mechanism for the antiviral effects of heme oxygenase? Hepatology DOI: 10.1002/hep.23921.
     
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