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Fluorescent Dyes  >  Enzyme Detection Reagents  >>  360 MMP FRET Substrate IV

Product Name 360 MMP FRET Substrate IV
Size 5 mg
Catalog # AS-27099
US$ $291
Purity % Peak Area By HPLC ≥ 95%

This substrate has a fluorescent Trp residue in subsite P2 and a dinitrophenol (Dnp) quenching group at the N-terminus. The quenching of the Trp fluorescence in the intact substrate is relieved upon hydrolysis of the P1-P1 bond, giving rise to a continuously recording fluorescence assay. The residues placed in subsites P3-P1 and P3 is optimized for MMP-3, while Arg is placed in P4 to enhance solubility. The substrate hydrolysis rate rivals or exceeds those of the corresponding natural protein substrates or other synthetic peptides. The solubility of this substrate in assay buffer exceeds the Km value for each reaction, allowing accurate determination of the kinetic parameters. Abs/Em = 280/360 nm.

Storage -20C
References Ref: Finch-Arietta, M. et al. Agents Actions 39, C189 (1993); Netzel-Arnett, S. et al. Anal. Biochem. 195, 86 (1991).
Molecular Weight 1152.3
(One-Letter Code)
(Three-Letter Code)
Dnp - Pro - Tyr - Ala - Tyr - Trp - Met - Arg - OH
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