Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. This recombinant human MMP-14 has a purity of >95% on SDS-PAGE and should be activated before experiments. It can be used in FRET-based enzyme activity assay or served as positive control for zymography, Western blot, IP, and ELISA.