IMG-EN-AS-72124-01.jpg
Assay-kits

SensoLyte® AFC Plasmin Activity Assay Kit Fluorimetric - 1 kit

$461.00
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  • Cat.Number : AS-72124
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Plasmin belongs to the family of serine proteases. It plays a key role in fibrinolysis by dissolving fibrin in blood clots. Besides fibrinolysis, plasmin is also involved in such physiological and pathological processes as wound healing, liver repair, and the maintenance of liver homeostasis.
The SensoLyte® AFC Plasmin Activity Assay Kit provides a convenient assay for high throughput screening of plasmin inhibitors and inducers or for continuous assay of plasmin activity. The assay utilizes a fluorogenic peptide, which upon enzyme cleavage releases the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore. The fluorescent signal can be measured at Ex/ Em =380 nm/500 nm. This assay can detect picogram level of plasmin activity.

Specifications

Packaging
Kits components
  • Component A: Plasmin substrate, Ex/Em=380 nm/500 nm upon cleavage: 3 mM, 50 µL Component B: AFC, fluorescence reference standard, Ex/Em=380 nm/500 nm: 3 mM, 10 µL Component C: Human plasmin: 250 µg/mL, 10 µL Component D: 2X Assay Buffer: 10 mL Component E: Plasmin Inhibitor: 1 mM, 10 µL Component F: Stop Solution: 5 mL
Chemistry
UniProt number
  • P00747
Properties
Absorbance (nm)
  • 380
Emission (nm)
  • 500
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Component D and F can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 0.8 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Genome-wide protein QTL mapping identifies human plasma kallikrein as a post-translational regulator of serum uPAR levels

FASEB J . 2013 Nov 18 ; 28 923 | DOI : 10.1096/fj.13-240879

  • M.A. Portelli

Identification of a specific haptoglobin C‐terminal fragment in arthritic synovial fluid and its effect on interleukin‐6 expression

Immunology . 2013 Sep 01 ; 140 133 | DOI : 10.1111/imm.12125

  • H.J. Park

Intravenous and oral administrations of DD2 [7-Amino-2-(sulfanylmethyl)heptanoic acid] produce thrombolysis through inhibition of plasma TAFIa in rats with tissue factor-induced microthrombosis

Thromb Res . 2012 Jul 12 ; 130 e222 | DOI : 10.1016/j.thromres.2012.06.018

  • T. Sasaki

uPAR regulates bronchial epithelial repair in vitro and is elevated in asthmatic epithelium

Thorax . 2011 Dec 03 ; 67 477 | DOI : 10.1136/thoraxjnl-2011-200508

  • C.E. Stewart

Urinary plasmin inhibits TRPV5 in nephrotic-range proteinuria

J Am Soc Nephrol . 2012 Sep 27 ; 23 1824 | DOI : 10.1681/ASN.2011111126

  • K. Tudpor

Reactive Center Loop (RCL) Peptides Derived from Serpins Display Independent Coagulation and Immune Modulating Activities.

J Biol Chem . 2015 Nov 30 ; 291(6) 2874 | DOI : 10.1074/jbc.M115.704841

  • S. Ambadapadi

Nano-zymography Using Laser-Scanning Confocal Microscopy Unmasks Proteolytic Activity of Cell-Derived Microparticles

Theranostics . 2016 Mar 02 ; 6(5) 610 | DOI : 10.7150/thno.13757

  • A. Briens
  • et al