Host: Rabbit Clone Number: N/A Isotype: Rabbit IgG Species Reactivity: zebrafish Application: Western blotting Product Description: This Rabbit Polyclonal anti-Cyclin B1 antibody is supplied as an epitope affinity purified Rabbit IgG, 50 µg in 250 µl of MOPS buffer (pH 7.4) containing 50% glycerol and 0.05% sodium azide. Immunogen: Peptide derived from internal region of zebrafish cyclin B1 protein Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator. WB: 1:500-1000 dilution
Figure 1. Western blot of zebrafish embryonic cell lysate (5 hpf) probed with the anti-zebrafish Cyclin-B1 (IN) antibody (Cat# 55443). An immunoreactive band was detected at ~45 kDa (A). The reaction was blocked when the antibody was pre-incubated with the immunizing peptide (B).