Host: MouseClone: 4D9 Isotype: IgG1 Concentration: 1 mg/ml Species Reactivity: All species Specificity: 5-hmC (5-Hydroxymethylcytosine) Epitope: Modified 5-hydroxymethylcytosine found in DNA of vertebrates Purity: >95% (Purified IgG fractions prepared by affinity chromatography using Protein A)Storage Buffer: Phosphate Buffer Saline 10 mM, 0.15M NaCl, pH 7.4 Storage Instructions: Store aliquoted at -20C. Avoid repeated freeze/thaw cyclesValidated Applications: These are suggested amounts/dilutions. Optimal condition should be determined by the end user.Hydroxymethyl DNA Immunoprecipitation: 1-3 µg per IPIF: 1:500ELISA: 1:1000Dot Blot: 1:2000RELATED PRODUCTS:Monoclonal Antibody against 5 - Methylcytosine (available in 3 sizes – 0.1, 0.5 & 1 mg)Starter Kit: 50 ug each of 5-Methylcytosine (5-mC) mAb, clone 33D3 and 5-hydroxymethylcytosine (5-hmC) mAb, clone 4D9Figure 1. Dot blot of 5-hmC mAb (4D9) with DNA standard, Cytosine (C), 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC).Figure 2. Titer of 5-hydroxymethylcytosine (5-hmC) monoclonal antibody (4D9). Estimated titer is 0.05 µg/ml.