Existing Account

Please login first to complete purchase/ quotation request, view custom order reports, or create favorites list.

Customer ID:
Stay Logged In

Forgot your Customer ID or Password?
New Account

Don't have an account with us yet? Please set up an account to place order or obtain customer services.

Assay Kits  >  Secretase Assay Kits  >>  SensoLyte ® 520 β-Secretase Assay Kit *Fluorimetric*

Product Name SensoLyte ® 520 β - Secretase Assay Kit *Fluorimetric*
Size 1 kit
Catalog # AS-71144
US$ $529

BACE1 (β-Secretase) is a membrane-anchored aspartic protease existing in acidic subcellular vesicles. It is a key player in producing β-amyloid peptides through proteolytic cleavage of the β-amyloid precursor protein (APP). Accumulation of neurotoxic β-amyloid peptide is seen in senile plaques in the brains of patients with Alzheimer’s disease (AD), an age-related cognitive disorder. Therefore, targeting β-secretase is considered a potential therapeutic strategy for the prevention and treatment of AD.

The SensoLyte® 520 BACE1 (β-Secretase) Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of secretase inhibitors. It contains a QXL® 520/ HiLyte™ Fluor 488 FRET substrate. The sequence of this FRET peptide is derived from the β-secretase cleavage site on the Swedish APP mutation. This mutation causes the enhancement of β-secretase to process APP, resulting in an early onset of AD. Active β-secretase cleaves FRET substrate into two separate fragments resulting in the release of HiLyte™ Fluor 488 fluorescence that can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of HiLyte™ Fluor 488 is less interfered by the autofluorescence of components in biological samples and test compounds. Kit Size: 100 assays (96-well plate)

SensoLyte® 520 BACE2 Activity Assay *Fluorimetric*
SensoLyte® 520 ADAM10 Activity Assay Kit *Fluorimetric*
SensoLyte® 520 Neprilysin Activity Assay Kit *Fluorimetric*
SensoLyte® Cathepsin Assays *Fluorimetric*
SensoLyte® Anti-α-Synuclein (Human, Mouse, Rat) ELISA Kit *Colorimetric*
SensoLyte® Anti-β-Amyloid (1-40) & (1-42), Human, Mouse/ Rat ELISA Kits *Colorimetric*
Recombinant human α & ß-Synucleins
β-Amyloid Peptides - Wide Selection

Product Spotlight: Tau Products - Peptides and Recombinant Proteins

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Product Citations Boddapati, S. et al. (2011). Inhibiting β-secretase activity in Alzheimer's disease cell models with single-chain antibodies specifically targeting APP. J Mol Biol 405, 436. doi: 10.1016/j.jmb.2010.10.054.

Mowrer, KR. & MS. Wolfe (2008). Promotion of BACE1 mRNA alternative splicing reduces amyloid β-peptide production. J Biol Chem 283, 18694. doi: 10.1074/jbc.M801322200.
  < Back